华北农学报2012，Vol.27Issue(3)：91-95,5.
梅花PmCBF基因的克隆及表达
Cloning and Expression of PmCBF Gene from Prunus mume
摘要
Abstract
The CBF transcription factor gene plays an important role in cold acclimation of plants. In this stud-y, a putative sequences of CBF gene was identified in the Genome Datebase of Prunus by underlying the electronic cloning technique , using sequence of Prunus avim in formation. A cDNA clone and gene clone encoding CBF gene were isolated from the cDNA and gDNA prepared from leaf of Prunus mume by the gene specific RT-PCR and PCR amplification. The gene was 711 bp which are capable of encoding protein of 225 amino acids(aa) .respectively. Se-queces analysis showed that the PmCBF gene owns the characteristics of knowned CBF transcriptional factors, which contains an highly conserverd AP2/EREB DNA-binding domain and two special short amino acids sequences. Ho-mology analysis indicate that the nucleotide sequences of the PmCBF gene were highly similar with those of Prunus avim,Prunus tella etc,both were up to 90% similarity, while the deduced amino acids sequences of it shared 100% identity with Prunus avim. The relative real-time PCR analysis showed that the expression of the PmCBF gene accord with other species after exposed to 4℃ . The expression of the PmCBF gene increased at the beginning and arrived at the peak after 4 hours. So we can conclude that the PmCBF gene is induced by low temperature stress.关键词
梅花/CBF转录因子基因/实时荧光定量PCRKey words
Prunus mume/CBF transcriptional factor gene/Relative real-time PCR分类
农业科技引用本文复制引用
张杰,杨炜茹,郝瑞杰,张启翔..梅花PmCBF基因的克隆及表达[J].华北农学报,2012,27(3):91-95,5.基金项目
国家"863"计划项目(2011AA100207) （2011AA100207）
