华北农学报2012，Vol.27Issue(3)：96-101,6.
沙冬青脱水素基因转化紫花苜蓿的研究
Research on Transformation of DHN Gene from Ammopiptanthus mongolicus into Alfalfa(Medicago sativa L.)
摘要
Abstract
In order to obtain transgenic plants of resistant drought,by comparative analysis on the different ex-plant tissues, concentrations of Agrobacterium and kanamycin, time of inoculation and coculture , etc , an Agrobacteri-um-mediated transformation for an alfalfa variety Zhongmu No. 2 was established. On the basis of the transformation, dehydrin of Ammopiptanthus mongolicus was transformated into genome of Zhongmu No. 2 by A. tumefaciens- mediated. The results showed that cotyledon and hypocotyl was the best explant for callus induction and transformation. The optimal kanamycin concentration for selection of resistant callus and transgenic plant was 25 mg/L in the selection medium. The explants and bacteria was cocultured for 5 d. One hundred and twenty-six independent resistant plants were obtained, 30 plants were confirmed to be transgenic by PCR amplification of AmDHN gene and 355 gene.关键词
紫花苜蓿/遗传转化/脱水素基因(AmDHN)Key words
Alfalfa/ Genetic transformation/ Dehydrin分类
生物科学引用本文复制引用
聂利珍,郭九峰,孙杰,孙国琴,刘永志..沙冬青脱水素基因转化紫花苜蓿的研究[J].华北农学报,2012,27(3):96-101,6.基金项目
内蒙古农牧业科学院科技创新基金项目(2011-CXJJM01) （2011-CXJJM01）
国家牧草产业技术体系(CARS-35) （CARS-35）
