华北农学报2012，Vol.27Issue(3)：231-233,3.
小麦植株中啶虫脒残留的气相色谱分析方法的建立
Determination of Acetamiprid Residues in Wheat Plants by GC-NPD
摘要
Abstract
In order to confirm the reasonable acetamiprid dose to control later wheat pests, the analysis method of acetamiprid residue in wheat plants has been studied systematically. And a method for determination acetamiprid residue in wheat plants have been developed by solid phase extraction ( SPE ) coupled with gas chromatography. Wheat plant samples was firstly shacking extracted in acetonitrile, then purified and concentrated by florisil solid phase extraction cartridge. The detection of acetamiprid was performed by GC-NPD using capillary program rising temperature and NPD detector. Under the optimal conditions,the retention time of acetamiprid was 10.01 minutes, the calibration curves showed good linearity ( Y = 3 678. 37 Χ - 3 595. 04, R2 =0.999 8) from 0. 1 mg/L to 200 mg/L concentration of acetamiprid, the recoveries at the three spiked concentration levels ranged from 82. 73% to 119.53% ,and the relative standard deviations( RSDs) for blank sample spiked with standard were in the range of 1.31% to 4. 73% . The detection limit of acetamiprid was 0. 74 ng and the lowest concentration detected was 0. 018 mg/kg in wheat plants. The results indicated that the method is simple, efficient, economical and reliable,and it's suitable for the determination of acetamiprid residues in wheat plants.关键词
小麦植株/农药残留/啶虫脒/气相色谱法/检测Key words
Wheat plants/Pesticide residues/Acetamiprid/Gas Chromatography/Determination分类
农业科技引用本文复制引用
李广领,王建华,陈锡岭,武予清,李金丽,肖娜娜..小麦植株中啶虫脒残留的气相色谱分析方法的建立[J].华北农学报,2012,27(3):231-233,3.基金项目
农业部农业公益性行业科研专项(200803002-6) （200803002-6）
