郑州大学学报（医学版）2012，Vol.47Issue(4)：429-431,3.DOI:10.3969/j.issn.1671-6825.2012.04.001
靛玉红甲肟对食管癌EC9706细胞增殖、凋亡的影响
Effects of indirubin-3'-monoxime on proliferation and apoptosis of human esophageal cancer EC9706 cells
摘要
Abstract
Aim: To explore the effects of indirubin -3 ' -monoxime on proliferation and apoptosis of esophageal cancer EC9706 cellsM ethodS:EC9706 cells were treated with 0,1,5 ,and 10 μLmol/L indirubin-3 ' -monoxime for 12,24 and 48 h,then the proliferative inhibition rate was measured by MTT assay ES706 cells were treated with 10 μLmol/L indirubin-3 ' -monoxime for 0,24,48 , and 72 h, then the apoptosis rate was measured by flow cytometry . EC9706 cells were treated with 0,1,5 , and 10 μmol/L indirubin-3 ' -monoxime for 48 h,then the expression of Sox-2 mRNA was analyzed by RT-PCR. ReSUltS; Indirubin-3 ' -monoxime markedly inhibited the proliferation of EC 9706 cells in a dose-and time-dependent manner(Fconcentration =135.237,Ftime =96.488,Finteraction=189.544,P <0.001). The apoptosis rate increased in a time-dependent manner (F = 195. 350 ,P <0. 001) ,and Sox-2 mRNA was downregulated in a dose-dependent manner (F = 87. 552 , P <0001 ) after the treatment by indirubin-3- monoxime. Conclusion:Indirubin-3 ' -monoxime could inhibit the proliferation and induce apoptosis of EC9706 cells through down regulating Sox-2 mRNA expression.关键词
食管肿瘤/靛玉红甲肟/Sox-2/增殖/凋亡/EC9706Key words
esophageal neoplasm /indirubin-3 ' monoxime /Sox-2/ proliferation /apoptosis/ EC9706分类
医药卫生引用本文复制引用
刘晓莉,刘怀民,高启龙,陈小兵,张成娟,杨峰..靛玉红甲肟对食管癌EC9706细胞增殖、凋亡的影响[J].郑州大学学报（医学版）,2012,47(4):429-431,3.基金项目
河南省医学科技攻关计划基金资助项目 2011020156 （）
