郑州大学学报（医学版）2012，Vol.47Issue(4)：459-463,5.DOI:10.3969/j.issn.1671-6825.2012.04.011
实验性胃溃疡大鼠自愈期间胰腺组织三叶因子3水平的变化
Changes of TFF3 expression in pancreatic tissue during self healing of rats with experimental gastric ulcer
摘要
Abstract
Aim: To study the changes of trefoil factor 3 (TFF3 ) expression during the self healing of gastric ulcer ( GU). Methods: A total of 30 SD rats were allocated into 5 groups ; the control group and GU1, GU4, GU10, GU23 groups , and 6 in each group. The rats in GUl, GU4 , GU10 , GU23 groups were made the GU model induced by injecting acetic acid into submucosa of gastric anterior wall , and the following indexes were detected at 1,4,10, and 23 days after the injection , respectively. The expressions of TFF3 and insulin in the pancreas were detected by immunohistochemistry SP method ,TFF3 mRNA was detected by RT-PCR, serum content of TFF3 was detected by ELISA. Results:There were significant differences in the expressions of TFF3 and insulin,TFF3 mRNA in the pancreas ,and serum content of TFF3 among the 5 groups (F was 9. 276 ,12. 362 ,166. 600 , and 21. 316 , respectively , P < 0. 001). The expressions of TFF3 in pancreas of GU4 , GU10 groups were high , in those of GU23 group decreased , but still higher than the control group (P < 0. 05 ). The changes of insulin was similar and positively related to TFF3(r =0. 826,P <0. 05) 4 d after the injection. The TFF3 mRNA expression increased from day 1,arrived the peak on day 4,decreased a little on day 10 ,and the level on day 23 was similar to day 1, but still higher than control group (P < 0. 05). Conclusion:TFF3 may participate in the self healing of GU by increasing transcriptional level and releasing to blood together with insulin .关键词
大鼠/胰腺组织/三叶因子3/胃溃疡/胰岛素Key words
rat/ pancreatic / trefoil factor 3 / gastric ulcer/ insulin分类
医药卫生引用本文复制引用
魏亚伟,吴靖芳,连雅君,张慧芹,蔡倩楠,王志勇,张静,任君旭..实验性胃溃疡大鼠自愈期间胰腺组织三叶因子3水平的变化[J].郑州大学学报（医学版）,2012,47(4):459-463,5.基金项目
河北省自然科学基金资助项目 C200700906 （）
