化工学报2012，Vol.63Issue(8)：2453-2459,7.DOI:10.3969/j.issn.0438-1157.2012.08.017
新型混合模式层析介质制备及用于牛初乳免疫球蛋白分离
New mixed-mode adsorbent and its application for separation of bovine colostrum immunoglobulin
摘要
Abstract
A new mixed-mode absorbent, named B-DVS-MI, with functional ligand 2-mercaptoimidazole was prepared and used for separation of immunoglobulin from bovine colostrums. There could exist hydrophobic, electrostatic and thiophilic interactions between B-DVS-MI and the adsorbate. Divinylsulfone activation and ligand coupling processes on the agarose beads were employed and optimized, resulting in activation density of 100 μmol · ml-1 and ligand density of 65 μmol · ml-1. The adsorption isotherm and kinetics for B-DVS-MI - bovine colostrum immunoglobulin system were measured and analyzed. The results showed that new adsorbent was of high capacity of immunoglobulin [127.6 mg · (g gel)-1], and of typical salt-independent properties. Its adsorption behaviors were significantly influenced by pH, so, with pH adjustment effective adsorption and elution could be achieved. The chromatographic conditions were optimized to separate effectively immunoglobulin from bovine colostrum powder, and product purity up to 95%. As a conclusion, bovine colostrum immunoglobulin could be effectively separated by mixed-mode adsorption chromatography, which could be a new way for comprehensive utilization of bovine colostrums.关键词
混合模式层析/2-巯基咪唑/二乙烯基砜活化/免疫球蛋白/牛初乳Key words
mixed- mode chroma tography/ 2-mercaptoimidazole/ divinylsulfone activation/ immunoglobulin/ bovine colostrums分类
化学化工引用本文复制引用
陈振明,林东强,姚善泾..新型混合模式层析介质制备及用于牛初乳免疫球蛋白分离[J].化工学报,2012,63(8):2453-2459,7.基金项目
项目资助:国家自然科学基金项目(21036005,20976154) （21036005,20976154）
新世纪优秀人才支持计划(NCET-08-0483). （NCET-08-0483）
