黑龙江八一农垦大学学报2012，Vol.24Issue(3)：41-44,4.
柱前衍生化高效液相色谱检测木糖液中葡萄糖含量
Determination of Glucose in Solution of Xylose by Pre-column Derivatization High Performance Liquid Chromatography
贾鹏禹 1孙蕊 2李良玉 1李洪飞 1张桂芳 1孙大庆 1于伟1
作者信息
- 1. 黑龙江八一农垦大学黑龙江省农产品加工工程技术研究中心,大庆163319
- 2. 黑龙江八一农垦大学动物科技学院
- 折叠
摘要
Abstract
The determination method of glucose in solution of xylose was established with pre-column derivatization HPLC in this paper. The content of glucose in solution of xylose was determined by RP-HPLC,and pre-column derivatization by 1-phenyl-3- methyl-5-pyrazolone （PMP）,and a ZORBAX Eclipse XDB-C18 （150 mmx4.6 mm ID,5μm）column was used,mobile phase was 0.1 mol.L-1 NH,Ac （pH=5.71）and flow rate was 1.0 mL.min-1 and UV detector at a wave length of 245 nm （BW 50 nm）.The calibration curve was linear in the mass concentration range of 2.5-50.0 mg. L-1, the LOD of method was 0.20 mg.L-1. The HPLC method is simple, convenient and sensitive.关键词
木糖液/葡萄糖/1-苯基-3-甲基-5-吡唑啉酮（PMP）/衍生化/HPLCKey words
solution of xylose/glucose/PMP/derivatization/HPLC分类
化学化工引用本文复制引用
贾鹏禹,孙蕊,李良玉,李洪飞,张桂芳,孙大庆,于伟..柱前衍生化高效液相色谱检测木糖液中葡萄糖含量[J].黑龙江八一农垦大学学报,2012,24(3):41-44,4.
