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Lactobacillus plantarum LY-78产苯乳酸发酵培养基优化的研究

李士龙 陈明霞 田雪娇 张丽萍

黑龙江八一农垦大学学报2012,Vol.24Issue(4):46-51,6.
黑龙江八一农垦大学学报2012,Vol.24Issue(4):46-51,6.

Lactobacillus plantarum LY-78产苯乳酸发酵培养基优化的研究

Optimization Study on Phenyllactic Acid Production Fermentation Medium by Lactobacillus plantarum LY-78

李士龙 1陈明霞 2田雪娇 1张丽萍3

作者信息

  • 1. 黑龙江八一农垦大学食品学院,大庆163319
  • 2. 黑龙江八一农垦大学食品学院,大庆163319 大庆油田技术培训中心
  • 3. 黑龙江八一农垦大学食品学院,大庆163319 黑龙江省农产品加工工程技术研究中心
  • 折叠

摘要

Abstract

Plackett-Burman design method was used to screen seven factors of affecting phenyllactic acid production by Lactobacillus plantarum LY-78.The results showed that:the addition amount of glucose,phenylalanine,Tween-80 was the key factor,on the basis of steepest ascent method approaching optimal response surface region,response curve method was used to research optimal level range of those 3 remarkable factors to determine that optimum was Glucose 24 g·L-1,phenylalanine 13 g·L-1 and Tween-80 7.4 mL·L-1,the output of phenyllactic acid was 2.13 g·L-1,which was 7.65 times than before optimization,higher than current phenyllactic acid strains.

关键词

苯乳酸/反相高效液相色谱/发酵培养基/响应面优化法

Key words

phenyllactic acid/RP-HPLC/fermentation medium/response surface methodology

分类

化学化工

引用本文复制引用

李士龙,陈明霞,田雪娇,张丽萍..Lactobacillus plantarum LY-78产苯乳酸发酵培养基优化的研究[J].黑龙江八一农垦大学学报,2012,24(4):46-51,6.

黑龙江八一农垦大学学报

1002-2090

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