黑龙江八一农垦大学学报2012,Vol.24Issue(4):46-51,6.
Lactobacillus plantarum LY-78产苯乳酸发酵培养基优化的研究
Optimization Study on Phenyllactic Acid Production Fermentation Medium by Lactobacillus plantarum LY-78
李士龙 1陈明霞 2田雪娇 1张丽萍3
作者信息
- 1. 黑龙江八一农垦大学食品学院,大庆163319
- 2. 黑龙江八一农垦大学食品学院,大庆163319 大庆油田技术培训中心
- 3. 黑龙江八一农垦大学食品学院,大庆163319 黑龙江省农产品加工工程技术研究中心
- 折叠
摘要
Abstract
Plackett-Burman design method was used to screen seven factors of affecting phenyllactic acid production by Lactobacillus plantarum LY-78.The results showed that:the addition amount of glucose,phenylalanine,Tween-80 was the key factor,on the basis of steepest ascent method approaching optimal response surface region,response curve method was used to research optimal level range of those 3 remarkable factors to determine that optimum was Glucose 24 g·L-1,phenylalanine 13 g·L-1 and Tween-80 7.4 mL·L-1,the output of phenyllactic acid was 2.13 g·L-1,which was 7.65 times than before optimization,higher than current phenyllactic acid strains.关键词
苯乳酸/反相高效液相色谱/发酵培养基/响应面优化法Key words
phenyllactic acid/RP-HPLC/fermentation medium/response surface methodology分类
化学化工引用本文复制引用
李士龙,陈明霞,田雪娇,张丽萍..Lactobacillus plantarum LY-78产苯乳酸发酵培养基优化的研究[J].黑龙江八一农垦大学学报,2012,24(4):46-51,6.