华南农业大学学报2012,Vol.33Issue(3):331-335,5.
印楝素诱导黑腹果蝇复眼发育异常的形态学研究
Abnormal Morphological Development of Drosophilia melanogaster Compound Eyes Induced by Azadirachtin
摘要
Abstract
The morphological characters of compound eyes dysplasia in Drosophilia melanogaster induced by azadirachtin was investigated. Flies were allowed to lay eggs on food mixture with 2 mg/kg of azadirachtin on which the resultant larvae and adults were fed and sampled for observation at different developmental stages. The images of stereo microscope and scanning electron microscope showed that adult eyes treated with 2 mg/kg of azadirachtin became smaller in size, irregular in ommatidia organization, rough on surface, duplicated, bent and forked in interommatidial bristles. The ultrastructure of compound eyes was characterized by transmission electron microscope. Results showed that chromatin condensation, phagocytic vacuole increase, nuclear membrane shrinkage, boundary fuzziness occurred in eye cells at the late third instar larvae, and they also showed that photoreceptors loss in different degrees at pupae and new adults. These results suggested that azadirachtin could induce abnormal morphological development in Z). melanogaster compound eyes, which may gain a new insight into novel insect growth regulators.关键词
印楝素/黑腹果蝇/复眼/发育异常/形态Key words
azadirachtin/ Drosophila melanogaster/ compound eyes/ dysplasia/ morphology分类
农业科技引用本文复制引用
李海怡,任太军,刘海远,钟国华..印楝素诱导黑腹果蝇复眼发育异常的形态学研究[J].华南农业大学学报,2012,33(3):331-335,5.基金项目
国家自然科学基金(30971944) (30971944)