华南农业大学学报2012，Vol.33Issue(3)：388-392,5.
不同电激活参数及CB处理对猪卵母细胞孤雌发育的影响
Effects of Different Electrical Stimulation Parameters and Cytochalasin B Treatment on Parthenogenetical Development of Porcine Oocytes
摘要
Abstract
Oocytes were collected from porcine ovaries, cultured in vitro for 42 -44 h, and cumulus cells were removed from oocytes. Matured oocytes were selected for parthenogenetical activation. The effect of different field strengths ( FSs) > pulse durations ( PDs) , electrical pulse numbers ( EPNs) , and the influ-ence of cytochalasin B treatment on development of parthenogenetically activated porcine oocytes were investigated. In the first experiment, 4 different PDs (80,100,120,140 μs) were applied with a single or two 0. 8 kV · cm-1 pulse(s) to activate the oocytes. The results showed that a single 100 μs pulse of 0. 8 kV/cm resulted in the highest blastocyst rate of (42. 22±5. 38)% , but it was not statistically different from the blastocyst rates of other groups ( P > 0. 05). In the second experiment, 4 different FSs (0. 8, 1.0,1.2,1.4 kV· cm-1) were used with a single or two 100 μs pulse(s) to stimulate the oocytes. The highest blastocyst rate [ (44. 04±0. 68)% ] was observed in the group applied with single 100 μs pulse of 1.4 kV·cm-1, but it was not statistically different from the blastocyst rates of other groups (P > 0.05). In the third experiment, 4 different PDs (80,100,120,140 μs) were used with a single 0. 8 kV· cm -2 or 1.4 kV · cm -1 pulses to activate the ooeytes. The biastocyst rate of the group treated with a single 80 μs pulse of 1.4 kV·cm-1 reached (54. 60±2. 86)% , which was significantly higher than that of other groups (P < 0. 01). In the fourth experiment, one group of oocytes was stimulated only by a single 80 ΜS pulses of I. 4 kV· cm-1 , while the other groups was activated with a single 80 ΜS pulses of L4 kV ·cm-1 followed by 4 h-cytochalasin B treatment. The results indicated that the blastocyst rate of the latter group was (54. 07±3. 12)% , significantly higher than that of the former group (P <0. 01 ). The above results suggested that EPN had no significant effect on parthenogenetical development of porcine oocytes, and 4 h-cytochalasin B treatment to the oocytes after the electrical stimulation by a single 80 μs pulses of 1. 4 kV·cm-1 resulted in a high parthenogenetical blastocyst rate.关键词
猪卵母细胞/囊胚率/孤雌激活Key words
porcine oocyte/ blastocyst rate/ parthenogenetical activation分类
农业科技引用本文复制引用
石俊松,罗绿花,周荣,周秀,麦然标,李紫聪,吴珍芳..不同电激活参数及CB处理对猪卵母细胞孤雌发育的影响[J].华南农业大学学报,2012,33(3):388-392,5.基金项目
国家科技重大专项(2011ZX08006) （2011ZX08006）
