湖南中医药大学学报2012，Vol.32Issue(3)：27-30,4.DOI:10.3969/j.issn.1674-070X.2012.03.008.027.04
珍稀濒危药材铁皮石斛组培快繁关键技术研究
Study on key technology of rapid culture and propagation for Dendrobium candidum as rare and endangered medicinal materials
摘要
Abstract
Objective To study the key technology of tissue culture and rapid propagation for Dendrobium candidum,a kind of rare and endangered medicinal materials.Methods The stem section of Dendrobium candidum was used as test materials to compare the effects of plant hormones concentration,artificial light and the seedling acclimatization method on tissue culture and rapid propagation of Dendrobium candidum and then established a set of industrialized breeding technical system for tissue culture and rapid propagation.Results (1) The best culture medium for PLBs induction: MS+BA 2.5 mg/L+IBA 0.2 mg/L; for differentiation: MS+ BA 1.5 mg/L+NAA 1.2 mg/L+5% banana juice; for both seedling and rooting: MS+NAA0.15 mg/L+ 5% banana juice,MS+NAA0.20 mg/L+7.5% bananna juice and MS+NAA0.25 mg/L 10% banana juice(Addition in different phases).(2)Artificial light: Specific spectral tissue energy-saving lamps.(3) The seedling acclimatization method: Hydroponics acclimatization.Conclusion Through the above technical application,the cycle of seedlings cultivation are shortened,the difficulties from seedling cultivation to scale production are settled,the method is simplified and production cost is reduced.关键词
铁皮石斛/组培快繁Key words
Dendrobium candidum/tissue culture and rapid propagation分类
医药卫生引用本文复制引用
赵兴兵,吴维佳,庞璐,蔡光先..珍稀濒危药材铁皮石斛组培快繁关键技术研究[J].湖南中医药大学学报,2012,32(3):27-30,4.基金项目
国家现代中药发展专项项目(发改办高技[2011]51号)资助. （发改办高技[2011]51号）
