湖南中医药大学学报2012，Vol.32Issue(7)：19-22,40,5.DOI:10.3969/j.issn.1674-070X.2012.07.006.019.04
黄芪总苷和三七总皂苷配伍对小鼠脑缺血再灌注脑组织能量障碍的影响
Effects of Astragalosides and Panax notoginseng saponins combination on energy metabolism of brain tissues after cerebral ischemia-reperfusion in mice
摘要
Abstract
Objective To investigate the effects of Astragalosides(AST) and Panax notoginseng saponins (PNS) combination on energy metabolism disturbance after cerebral ischemia -reperfusion in mice. Methods C57BL/6 mice were randomly divided into 6 groups as follows: sham-operated group,model group,Astragalosides group(AST,110 mg/kg·d,ig),Panax notoginseng saponins group (PNS,115 mg/kg·d,ig) ,AST combined with PNS group (AST 110 mg/kg·d+PNS 115 mg/kg·d.ig) and 3-methyl-1-phenyl-2-pyrazoline-5-one group (edaravone,8 mg/kg·d,ip). All mice were administered respectively and lasted for 4 d. After the last treatment for 1 h, cerebral ischemia-reperfu-sion injury model was established. Then, the contents of adenylate(ATP,ADP,AMP) in brain tissues were measured and the activities of Na+-K+-ATPase were evaluated. Results AST combined with PNS exhibited synergistic inhibition effects on the decrease of ATP and ADP contents, total ade- nine nucleotides (TAN) and ATPase activities (all P<0.01),and exhibited addition inhibition effects on the decrease of energy charge (EC) (P <0.05). Conclusion These results suggest that AST combined with PNS significantly improve cerebral energy metabolism dysfunction after is-chemia-reperfusion. The underlying mechanism may be that AST and PNS synergistically promote the supply and generation of energy, improve the energy charge, enhance Na*-K*-ATPase activity in brain tissues.关键词
黄芪总苷/三七总皂苷/配伍/脑缺血再灌注/能量代谢障碍/小鼠Key words
Astragalosides/ Panax notoginseng saponins/ combination/ cerebral ischemia-reperfusion/ energy metabolism dysfunction/ mouse分类
医药卫生引用本文复制引用
黄小平,谭华,刘文龙,邓常清..黄芪总苷和三七总皂苷配伍对小鼠脑缺血再灌注脑组织能量障碍的影响[J].湖南中医药大学学报,2012,32(7):19-22,40,5.基金项目
湖南省教育厅重点资助项目(08A050). （08A050）
