湖南中医药大学学报2012，Vol.32Issue(7)：34-36,49,4.DOI:10.3969/j.issn.1674-070X.2012.07.010.034.03
mRNA差异显示法筛选黄花石蒜中加兰他敏合成相关基因
Screening of the gene related to galantamine biosynthesis of Lycoris aurea Herb.by mRNA differential display
摘要
Abstract
Objective To isolate and clone the gene related to galantamine biosynthesis of Lycoris aurea Herb. Methods The specially expression cDNA fragments of the gene related to galantamine biosynthesis were screened by differential display polymerase chain reaction(DD-PCR) after extracting the total RNA from the pistils tissues and the scape tissues of the Lycoris aurea Herb cropped in July which had differential contents of the galantamine. Then these fragments were separately cloned to plasmid PMD19 -T and subsequently underwent sequence analysis and homogenous comparison. Results 44 differential display bands were found in gene expression between the pistils tissues and the scape tissues of the Lycoris aurea Herb. According to the sequence analysis and homologenous comparison, two of them were confirmed to related with galantamine biosynthesis, one of them was possible related with galantamine transport regulation. Conclusion The three homologous sequences of Lycoris aurea screened by DD-PCR provide strong basis of galantamine synthetic pathways.关键词
黄花石蒜/加兰他敏/mRNA差异显示技术/生物信息学Key words
Lycoris aurea Herb/ galantamine/ mRNA DD-PCR/ bioinformatics分类
医药卫生引用本文复制引用
唐金凤,鲁耀邦,桂柳姿..mRNA差异显示法筛选黄花石蒜中加兰他敏合成相关基因[J].湖南中医药大学学报,2012,32(7):34-36,49,4.基金项目
湖南省自然科学基金资助项目(10JJ2024) （10JJ2024）
湖南省教育厅科研基金资助项目(07A052). （07A052）
