湖南中医药大学学报2012，Vol.32Issue(7)：50-53,4.DOI:10.3969/j.issn.1674-070X.2012.07.014.050.04
HPLC同时测定菟丝子不同炮制品中5种主要活性成分含量
Determination of five main active ingredient content in different processed products of Cuscuta chinensis by HPLC
摘要
Abstract
Objective To establish the method for quantitative analysis of chlorogenic acid, hyperoside, quercetin, kaempferol and isorhamnetin in the Cuscuta chinensis different processed products by HPLC. Methods The Kromasil C18 column (250 mm×4.6 mm,5 μm) was used with methanol-0.4% phosphoric acid as mobile phase. Under the condition of gradient elution, the flow rate was 1.0 mL/min, the column temperature was 30℃ and the detection wavelength was 360 nm. Results Baseline separation was obtained separately for the five kinds of active ingredients and there was a good linear relationship (r≥0.999 7). The average recoveries were 98.4%~100.7% and RSD≤2.4%(n=6). Conclusion The established method in this study is sensitive,reliable and available for the quality control of the different processed products of Cuscuta chinensis.关键词
菟丝子/绿原酸/金丝桃苷/槲皮素/HPLCKey words
Cuscuta chinensis/ chlorogenic acid/ hyperoside/ quercetin/ HPLC分类
医药卫生引用本文复制引用
肖岚,杨梓懿,石继连..HPLC同时测定菟丝子不同炮制品中5种主要活性成分含量[J].湖南中医药大学学报,2012,32(7):50-53,4.
