湖南农业大学学报（自然科学版）2012，Vol.38Issue(4)：354-359,6.DOI:10.3724/SP.J.1238.2012.00354
油菜原花色素合成途径基因的克隆及进展
Advance in cloning of genes for proanthocyanidin biosynthesis from oilseed Brassica species
摘要
Abstract
Proanthocyanidins are the end products of flavonoid biosynthesis and the gene regulatory network of flavonoid biosynthesis has been fully uncovered in such species as Arabidopsis thaliana and grape whose genome has been sequenced. The accumulation of proanthocyanidins in seed coat is associated with seed color, seed dormancy and seed longevity and influences color and oil content of oilseed Brassica seeds. This review summarized progress in cloning of genes for proanthocyanidin biosynthesis by map-based cloning, homology cloning or by in silico cloning, analyzed the relationship between cloned genes for proanthocyanidin biosynthesis and seed coat color in oilseed Brassica species, proposed a new comprehensive approach to obtain full-length sequence of various gene copies by screening of bacterial artificial chromosome(BAC) library using publicly released genome sequences of Brassica napus and B. rapa and listed the copies of genes for proanthocyanidin biosynthesis cloned by the authors using the proposed method in order to uncover the gene regulatory network for proanthocyanidin accumulation in oilseed Brassica species.关键词
油菜/原花色素合成/基因克隆Key words
Brassica/ proanthocyanidin biosynthesis/ gene cloning分类
农业科技引用本文复制引用
刘忠松,孙东红,刘显军,官春云..油菜原花色素合成途径基因的克隆及进展[J].湖南农业大学学报（自然科学版）,2012,38(4):354-359,6.基金项目
国家自然科学基金项目(31101176) （31101176）
教育部博士点专项科研基金项目(200805370003) （200805370003）
湖南省研究生科研创新基金项目(CX2010B307) （CX2010B307）
