湖南农业大学学报（自然科学版）2012，Vol.38Issue(4)：404-407,4.DOI:10.3724/SP.J.1238.2012.00404
铅胁迫对茶条槭与五角槭的叶片叶绿素含量和膜脂过氧化及保护酶活性的影响
Effect of lead stress on chlorophyll content, membrane lipid peroxidation and protective enzyme activity in leaves of Acerginnala Maxim and Acer mono Maxim
摘要
Abstract
The membrane lipid peroxidation and protective enzyme activity in the annual seedlings of Acer ginnala Maxim and Acer mono Maxim were studied under lead stress through pot experiment. Treatments Pb100, Pb500, Pb1000, Pb2000 were added with 100, 500, 1 000 and 2 000 mg/kg of Pb(CH3COOV5H2O (pure Pb2-) respectively and the treatment with no Pb2+ addition was served as control(CK). Indexes of the leaf samples were determined 75 d after Pb addition. The results showed that chlorophyll a/b and proline contents decreased while relative conductivity, manlondialdehyde (MDA) content and the activity of peroxidase (POD) increased with the increase of lead concentration in soil. The activity of superoxide dismutase (SOD) rose firstly and then went down. The variations of these indexes in Acer mono were greater than those in Acer ginnala. Lead stress caused membrane lipid peroxidation in the plants and the peroxidation increased with increasing intensity of lead stress, which meanwhile caused anti-peroxidation effect. SOD played an important role and the change of POD was not obvious during the process of removing peroxidation products. In general, both Acer ginnala Maxim and Acer mono Maxim were tolerant to lead stress and the former had greater tolerance than the latter. Two kinds of trees could be used as landscape trees in lead polluted area.关键词
茶条槭/五角槭/铅胁迫/叶绿素/膜质过氧化/抗氧化酶/脯氨酸Key words
Acer ginnala Maxim/ Acer mono Maxim/ lead stress/ chlorophyll/ membrane lipid peroxidation/ antioxidant enzyme/ proline分类
农业科技引用本文复制引用
李亚藏..铅胁迫对茶条槭与五角槭的叶片叶绿素含量和膜脂过氧化及保护酶活性的影响[J].湖南农业大学学报（自然科学版）,2012,38(4):404-407,4.基金项目
国家自然科学基金项目(30371149) （30371149）
黑龙江省科技计划项H(GCOIKBKB213) （GCOIKBKB213）
