华西口腔医学杂志2012，Vol.30Issue(4)：350-355,359,7.DOI:10.3969/j.issn.1000-1182.2012.04.005
微小核糖核酸-21对人舌鳞状细胞癌细胞系生物学特性的影响
Biological effect of micro ribonucleic acid-21 on human tongue squamous cell carcinoma lines
摘要
Abstract
Objective To detect the biological influence to human tongue squamous cell carcinoma (TSCC) cells of micro ribonucleic acid-21 (miR-21). Methods Referring to mature miR-21 sequence, the sense and antisense oligonucleotide (sense-miR-21 and AS-miR-21) modified by 2'0-Me were designed to transfect into TSCC cells (Tca8113 and high metastasis cells) by liposome transfection technology, in order to establish an in vitro TSCC cell model. The expression changes of miR-21 in the transfected cells were detected with real-time fluorescence quantitative polymerase chain reaction (real-time PCR). The changes of cell proliferation, cell cycle, cell early apoptosis, cell migration and invasion capabilities were detected respectively by the technologies of methyl thiazolyl tetrazolium CMTT), flow cytometry, Annexin V cell early apoptosis assay, scratch assay and Transwell asbay, to check AS-miR-21's effect on the biologicalcharacteristics of human TSCC cell lines. Results For the TSCC cells, the antisense oligonucleotide of targeting miR-21 could effectively inhibit cell proliferation, promoted cell apoptosis, and inhibited the capability of cell's migration and invasion. Conclusion The expressions of miR-21 decrease after AS-miR-21 transfected into TSCC cells, and miR-21 can affect biological behavior of TSCC cells.关键词
舌鳞状细胞癌/微小核糖核酸-21/微小核糖核酸-21反义寡核苷酸/生物学特性Key words
tongue squamous cell carcinoma/ micro ribonucleic acid-21/ antisense-micro ribonucleic acid-21 oligonucleotide/ biological effect分类
生物科学引用本文复制引用
王寅,李龙江,徐克,吕品,郑文龙..微小核糖核酸-21对人舌鳞状细胞癌细胞系生物学特性的影响[J].华西口腔医学杂志,2012,30(4):350-355,359,7.基金项目
国家自然科学基金资助项目(30973345) （30973345）
