四川大学学报（医学版）2012，Vol.43Issue(3)：335-339,5.
姜黄素促卵巢癌耐药细胞株COC1/DDP凋亡机制探索
Study on Curcumin-induced Apoptosis in Ovarian Cancer Resistant Cell Lines COC1/DDP
摘要
Abstract
Objective To investigate the effect and possible mechanism of curcumin to induce apoptosis in ovarian cancer resistant cell lines COC1/DDP. Methods COC1/DDP cells were treated with different concentration of curcumin, with or without the combination of chemotherapy drugs cisplatin (DDP) and paclitaxel (PIX) for 48 hours. The growth inhibition rates of COCi/DDP cells were studied by MTT method, and the apoptotic ratios were measured with flow cytometry. The expression of phosphoinositide 3-kinase catalytic subunit (PI3KCA) mRNA was studied by RT-PCR in curcumin treated cells, DDP treated cells and their combination treated cells. Results After the treatment of different concentration of curcumin for 48 hours, the growth inhibition rates and the apoptotic rate of COCI/DDP cells were gradually increased accordingly with increasing curcumin concentration. Furthurmore, curcumin in combination with chemotherapy drug obtained higher inhibition rate and apoptosis rate than single chemotherapy drug did (P<0. 05). The expression of PI3KCA mRNA of COCI/DDP cells treated with curcumin combined DDP was much lower than that treated only with DDP (P<0. 05). Conclusion Curcumin can increase the apoptotic rate of COCI/DDP cells, so has synergistic effect on with chemotherapy drugs on the induction of cell apoptosis. Its possible mechanism may be related to the down-regulation of PI3KCA.关键词
卵巢癌/COC1/DDP/姜黄素/顺铂/细胞凋亡/磷脂酰肌醇3-激酶催化亚基Key words
Ovarian carcinoma/ COCI/DDP/ Curcumin/ Cisplatin/ Apoptosis引用本文复制引用
林琳,王平,赵晓兰..姜黄素促卵巢癌耐药细胞株COC1/DDP凋亡机制探索[J].四川大学学报（医学版）,2012,43(3):335-339,5.基金项目
四川省科技厅基金项目(编号07FG002-011)资助 （编号07FG002-011）
