四川大学学报（医学版）2012，Vol.43Issue(3)：358-361,4.
中药五倍子对不同根面龋损提取非胶原蛋白后再矿化的影响
Re-mineralization Ability of Galla Chinensis Extracts on Root Carious Lesions with or without Non-collagen Proteins
阙克华 1郭斌 2王博 3粱倩倩 3谢红慧3
作者信息
- 1. 天津医科大学口腔医院牙体牙髓科,天津300070
- 2. 中国人民解放军总医院口腔医学研究所,北京100853
- 3. 四川大学口腔疾病研究国家重点实验室,成都610041
- 折叠
摘要
Abstract
Objective To evaluate the re-mineralization ability of Galla Chinensis extracts (GCE) on two artificial carious lesions in bovine root de-mineralized in vitro. Methods Fourteen bovine root blocks were divided into two parts from buccal to lingual direction. The mesial blocks were treated with a demineralization solution and the distant blocks were treated with another demineralization solution. Two specimens from each group were selected randomly and examined with polarization microscope (PLM). After all blocks were demineralized, half surface of the demineralized zone was covered and the another half was treated with 0. 5 % NaCl to extract soluble dentin phosphate protein (S-DPP). Then all specimens were submitted to pH-cycling for one week. In the first four days, all specimens were treated with GCE for 21 h and with demineralization solution for 3 h. In the remaining three days, all specimens were treated with GCE. The re-mineralization ability of GCE on the specimens was evaluated by laser scanning confocal microscope (LSCM). Results There existed intact surface layers on subsurface lesions but no surface layers were produced on erosive lesions. The re-mineralization ability of GCE on erosive lesions improved significantly with the treatment of 0. 5% NaCl solution (P<0. 05). But it had no significant effect on subsurface lessions. Conclusion Extraction of S-DPP with 0. 5% NaCl can improve the re-mineralization ability of GCE on root caries with erosive lesions. This finding supports the proposition that Galla Chinesis may be a promising anti-caries natural medicine in the future.关键词
五倍子/根面龋/非胶原蛋白/再矿化Key words
Galla Chinensis extract/ Root caries/ Non-collagen protein/ Re-mineralization引用本文复制引用
阙克华,郭斌,王博,粱倩倩,谢红慧..中药五倍子对不同根面龋损提取非胶原蛋白后再矿化的影响[J].四川大学学报（医学版）,2012,43(3):358-361,4.
