四川大学学报（医学版）2012，Vol.43Issue(4)：513-516,4.
奥美拉唑对肝癌HepG2细胞增殖与凋亡的影响
Effects of Omeprazole on the Proliferation and Apoptosis of Hepatoma Cell Line HepG2
摘要
Abstract
Objective To investigate the effects of omeprazole (OME), a proton pump inhibitor, on the proliferation and apoptosis of human hepatoma cell line HepG2. Methods HepG2 cells were cultured to the logarithmic phase, and then treated with OME of different concentrations (10, 20, 40, 80, 160 mg/L) for 24 h or 48 h. Cell proliferation was evaluated by MTT assay, DNA synthesis was measured with 5 ethynyl-2'-deoxyuridine (Edu) fluorescent assay and the apoptosis of cells was measured by the Hoechst33342 assay. Results MTT assay showed that OME (40, 80 and 160 mg/L concentrations) could inhibit the proliferation of HepG2 cells for 24 h or 48 h treatment (P<0. 05) and 80 mg/L group has strongest effect. Compared with that of 24 h treatment, the same concentration of OME could inhibit HepG2 more significantly with 48 h treatment. After different concentrations of OME treatment for 24 h and then incubation with Edu for 2 h, compared with the control group, the proportion of Cells in S phase in 20, 40, 80, 160 mg/L groups decreased. Hoechst33342 staining demonstrated that treatment with OME (40,80,160 mg/L) for 24 h could significantly promote the cell apoptosis. Conclusion Omeprazole could inhibit human hepatoma cell line HepG2 cell proliferation and promote apoptosis.关键词
奥美拉唑/肝癌/增殖/凋亡Key words
Omeprazole/Hepatoma/Proliferation/Apoptosis引用本文复制引用
魏艳,梁宁林,朱永军,吴文超,刘小菁,杨丽..奥美拉唑对肝癌HepG2细胞增殖与凋亡的影响[J].四川大学学报（医学版）,2012,43(4):513-516,4.基金项目
四川省科技厅项目(No.2009FZ0097)资助 （No.2009FZ0097）
