四川大学学报（医学版）2012，Vol.43Issue(4)：578-583,6.
PEI2k-SPIO纳米颗粒标记大鼠骨髓间充质干细胞的体外磁共振成像
MR Imaging of Polyethylenimlne-superparamagnetic Iron Oxide Nanoparticle Labeled Bone Marrow Mesenchymal Stem Cells in vitro
摘要
Abstract
Objective To explore the optimal concentration of polyethylenimine-superparamagnetic iron oxide (PEI2k-SPIO) particles for labeling bone marrow mesenchymal stem cells (BMSCs) in vitro, then to demonstrate the imaging characteristics of the cells by 7. 0-T MR scanner. The lowest cell quantity and the optimal cell quantity detected on MR was observed. Methods Cells at 2nd passage were inoculated into the 6-hole plate with cover glass. The different concentrations of PEI2k-SP10 (5 μg/mL, 7 μg/mL, 10 μg/mL, 15 μg/mL, 20 μg/mL) were added into different holes, respectively. After labeled with different concentrations of PEI2k-SPIO, the Prussian blue stain was used for determining the labeling efficiency. MTT growth curves were used to identify the activity of BMSCs and to determine the optimal threshold of SPIO nanocomposite particles labeled the stem cells at different PEI2k-SPIO concentrations (7, 10, 15, 20 μg Fe/mL medium). To definite the lowest cells quantity and the optimal observable cells quantity on MR imaging, 1×106, 1×105, 1×104 and 1×103 cells labeled with optimal threshold of PEI2k-SPIO and 1×106 cells unlabeled suspended in 0. 2 mL agarose (10 g/L). respectively undergone MR scan. Results MTT growth curves showed the optimal threshold of PEI2k-SPIO labeled BMSCs was 7 μg/mL, which indicates has no adverse effects on the growth of stem cells. At the opimal concentration (7 μg Fe/mL), the lowest observable cell quantity of PE12k-SPIO-labeled cells for MRI was 1×104, and the optimal observable cell quantity was 1×106. Conclusion At the opimal concentration, adverse effect to stem cell activities had not be detected when were labeled with PEI2k-SPIO and the clearly image of MRI of labeled BMSCs could be obtained.关键词
骨髓间充质干细胞/超顺磁性氧化铁/磁共振成像Key words
BMSCs/Superparamagnetic iron oxide/Magnetic resonance imaging引用本文复制引用
陈荟竹,郭应坤,李真林,夏睿,张丽芝,侯江龙,郜发宝,艾华,宁刚..PEI2k-SPIO纳米颗粒标记大鼠骨髓间充质干细胞的体外磁共振成像[J].四川大学学报（医学版）,2012,43(4):578-583,6.基金项目
国家自然科学基金(No.81130027)、四川省科技支撑项目(No.2010SZ0173,2011SZ0108)、四川省卫生厅项目(No.100381)和成都市科技局项目(No.11DXYB111SF)资助 （No.81130027）
