华中师范大学学报（自然科学版）2012，Vol.46Issue(4)：468-472,5.
野桑蚕漆酶基因克隆、序列分析及转录表达谱研究
Cloning, sequence analysis and transcriptional expression profiles of Bombyx mandarina Laccase gene
摘要
Abstract
The complemental deoxyribonucleic acid(cDNA)of Bombyx mandarina Laccase gene was cloned by reverse transcription-polymerase chain reaction (RT-PCR). The results showed that the cDNA with 2 317 bp in length contained an open reading frame (ORF) of 2 295 bp which encoded 764 amino acid residues, contains 8 exons and 7 in-trons, and a predicted molecular weight of 84340. 91 and isoelectric point of 6. 61. The deduced amino acid sequence had a high identity to the reported sequence of laccase2 from other lepidopterous insects and shared the typical structural features of laccase from other insects. The Laccase was only expressed in epidermis, head, midgut, and blood of B. mandarina by RT-PCR analysis. These results provide molecular basis for further studying the function of Laccase gene in B. mandarina.关键词
野桑蚕/漆酶基因克隆/序列分析/转录表达Key words
Bombyx mandarina/ Laccase gene cloning/ sequence analysis/ transcriptional expression分类
生物科学引用本文复制引用
张永亮,武安泉,盛东峰,张杰..野桑蚕漆酶基因克隆、序列分析及转录表达谱研究[J].华中师范大学学报（自然科学版）,2012,46(4):468-472,5.基金项目
河南省教育厅自然科学基金项目(2010A230016) （2010A230016）
周口师范学院博士科研启动基金资助项目 （）
周口师范学院校级重点学科建设经费资助项目. （）
