家畜生态学报2012，Vol.33Issue(2)：25-30,6.
家养水牛催乳素基因编码区结构特征
The Structural Characteristics in Coding Region of Prolactin Gene in Buffalo (Bubalus bubalus)
摘要
Abstract
The prolactin gene is considered as the important candidate genes that correlated with hastening growth,maintaining lactation and immunoregulation in water buffalo, but the structural characteristics in coding region of prolactin gene and the relationship between gene structure and production traits are still unclear. In the study, the splicing sequence variation in coding region of prolactin gene was examined in buffalo populations of swamp type and river type by using PCR-SSCP and DNA direct sequencing methods. The results showed that the mature peptide of prolactin consisted of 199 amino acid residues and the encoded protein was acidic, the usage rate of bases of A, T, C and G is 25. 96%, 22. 95%, 28. 14% and 22. 95%, respectively. The optimal codon accounted for 48. 21% of the total codon. The c. G192A?c C427T and c. C606T substitution was detected in swamp buffalo populations, which was synonymous and formed eight different haplotypes. A synonymous of c. C606T was detected in river buffalo populations and formed two different haplotypes. There was no expressive discrepancy in different haplotype in buffalo populations of swamp type and river type. Sequence analysis revealed the coding region of prolactin gene was highly conservative and not influenced in artificial selection, which might because of the functional constraints.关键词
家养水牛/催乳素基因/编码区/PCR-SSCPKey words
domesticated buffalo/ prolactin gene/ coding region/ PCR-SSCP分类
农业科技引用本文复制引用
袁峰,苗永旺,王绍卿,李大林,袁跃云..家养水牛催乳素基因编码区结构特征[J].家畜生态学报,2012,33(2):25-30,6.基金项目
云南省应用基础研究重点项目(2007C0003Z) （2007C0003Z）
国家自然科学基金项目(30660024) （30660024）
云南省应用基础研究计划面上项目(2006C0034M （）
2010ZC243) （）
国家高技术研究发展计划(863计划)项目(2008AA101001) （863计划）
国家自然科学基金项目30660024) （）
云南省教育厅科学研究基金项目(2010Y344) （2010Y344）
云南农业大学动物科学技术学院科学研究基金项目(DYK3P201104) （DYK3P201104）
