家畜生态学报2012，Vol.33Issue(4)：66-68,3.
温度与稀释液pH对猪精液常温保存效果的影响
Influence of Temperature and pH of Diluent on Preservation Efficiency of Boar Semen in Room Temperature
摘要
Abstract
To improve the preserving efficiency of boar semen in room temperature and ensure the fertilizing activity during semen dilution, the preserving conditions of boar semen, including temperature and pH of dilution buffer were optimized. The results showed that the survival time of the sperms in 15℃-20℃ was obviously longer than that in 21℃-25℃ (P<0. 05) when the sperms were stored in buffer I or Ⅱ with a respective motility rate of 50% and 30%. Similarly, the survival time in pH 6. 5-6. 9 was longer than that in pH 6. 0~6. 4 (P<0. 05). It suggested that the preserving conditions of boar semen was in 15℃-20℃ and in pH 6. 5-6. 9. Sperm acrosome staining results also showed that acrosome intact rate reached more than 96% after 48h semen preservation in optimum temperature and pH.关键词
猪精液/温度/pH/常温保存/稀释液Key words
boar semen/ temperature/ pH preservation in mormal temperature/ diluent分类
农业科技引用本文复制引用
林峰,杨婷,陈玉霞,韩雪蕾,高汉婷,李晓飞,高腾云..温度与稀释液pH对猪精液常温保存效果的影响[J].家畜生态学报,2012,33(4):66-68,3.基金项目
河南省重点科技攻关计划项目(82102130005) （82102130005）
河南省教育厅科学技术研究重点项目(12B230006) （12B230006）
