基础医学与临床2012，Vol.32Issue(8)：906-910,5.
Ang-(1-7)与Mas结合促进NIT细胞分泌胰岛素
The binding of Ang-(1-7) and Mas stimulates insulin secretion in NIT cell
摘要
Abstract
Objective To investigate the effects of Ang-(l-7) and Mas receptor on insulin secretion in NIT cell and their potential mechanism. Methods NIT cells were exposed to Ang-(l-7) of different concentrations (10~ ~ 10-3 mol/L) for 24 hours. Glucose stimulated insulin secretion by NIT cells was detected with ELISA. The full cDNA sequence of Mas, GLUT-2 and TGF-β1 were obtained from NIT cell line using RT-PCR. After NIT cells were exposed to 10-5 mol/L Ang-(l-7) for 48 hours, Mas, GLUT-2 and TGF-β 1 mRNA expression were estimated by real-time PCR; Meantime, the protein levels of the afore variables were detected by Western blot analy-sis. Results NIT islet cell line responded to extracellular Ang-(l-7) (10-8 -10-3 mol/L) with increased insulin secretion in a concentration dependent manner. The insulin secretion increased significantly in the presence of 10-5mol/LAng-(l-7) (8.86±0.53)mlU/Lcompared to control group (8, 06 ±0. 39)mlU/L) (P<0.05). In the experiments, compared to control group, the cells preincubated with 10-5 mol/L Ang-(l-7) were up-regulated in Mas gene and protein expression ( P < 0. 05 ) , and caused a significant stimulation of mRNA and protein expres-sion of GLUT-2 (P <0. 05). On the contrary, the result showed a reduction in TGF-βl mRNA and protein expression (P <0. 05). Conclusions The binding of Ang-( 1-7) and Mas can stimulate the NIT cells to secrete insulin, probably through its enhancing the ability to intake glucose and inhibit the progression of fibrosis.关键词
肾素血管紧张素系统,血管紧张素转换酶2/血管紧张素(1-7)/MasKey words
rennin-angiotensin system/ angiotensin-converting enzyme 2/ angiotensin-( 1-7) / Mas分类
医药卫生引用本文复制引用
张雪莲,杨金奎,尚军,王阳..Ang-(1-7)与Mas结合促进NIT细胞分泌胰岛素[J].基础医学与临床,2012,32(8):906-910,5.基金项目
国家自然科学基金(81070644) （81070644）
