基础医学与临床2012，Vol.32Issue(8)：926-929,4.
左炔诺孕酮下调survivin基因促进人子宫肌瘤细胞凋亡
Levonorgestrel induces apoptosis through down-regulation of survivin gene expression in human uterine leiomyoma cells
摘要
Abstract
Objective To explore the function of Survivin in levonorgestrel ( LNG) -induced apoptosis in cultured human uterine leiomyoma cells (UtLMC) in vitro. Methods AO/EB double staining was applied to discriminate the apoptotic cells from dead ones. The changes of survivin mRNA expressions in UtLMC after LNG treatment were detected by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) method. Furthermore, Western blot was used to determine survivin protein expression in LNG-induced UtLMC apoptosis. Results The result of AO/EB double staining showed that there were more viable apoptotic cells in LNG-treated groups than control group and the number of non-viable apoptotic cells and dead cells increased while the concentration of LNG elevated. Survivin expression in LNG-treated cells was decreased both at mRNA and protein levels according to RT-PCR and Western blot results. Conclusions Levonorgestrel treatment may induce a substantial apoptotic response in UtLMC, which was enhanced with the raise of levonorgestrel concentration. It may be due to the marked down-regulation of survivin gene expression.关键词
左炔诺孕酮/子宫肌瘤/细胞凋亡/survivinKey words
levonorgestrel/ uterine leiomyoma/ cell apoptosis/ survivin分类
医药卫生引用本文复制引用
仇黎丽,徐青,徐昌芬..左炔诺孕酮下调survivin基因促进人子宫肌瘤细胞凋亡[J].基础医学与临床,2012,32(8):926-929,4.基金项目
南京医科大学科技发展基金重点项目(2005NYDZD21) （2005NYDZD21）
南京市医学科技发展项目(YKK06080) （YKK06080）
