基础医学与临床2012，Vol.32Issue(8)：930-934,5.
人脐带间充质干细胞抑制同种异体脐血淋巴细胞的转化
Umbilical cord mesenchymal stem cells inhibit the transformation of lymphocytes from allogeneic umbilical cord blood
摘要
Abstract
Objective To study the biological characteristics of mesenchymal stem cells( MSCs) derived from human cord(UCMSCs) and the inhibitory effect on the transformation of lymphocytes from allogeneic Umbilical Cord Blood, Methods UCMSCs were isolated from umbilical cord. The effect of UCMSCs on the transformation of lymphocytes from allogeneic UGB was tested by MTT. Results The morphological feature of the third passage's UCMSCs ( P3-UCMSCs) displayed an uniform fibroblast-like phenotype. The doubling time of P3-MSCs was 51. 6 hours and expressed CD29 but not CD34. The histoehemical staining of P3-UCMSCs showed that PAS and NBE were positive but POX was negative. It indicated that the fibroblast-like cells induced and expanded from human cord in our experiment were MSCs; UCB-lymphocyte transformation was suppressed when UCMSCs were cultured together with UCB-lymphocytes and the suppression effect was related with UCMSCs numbers plated in eath well, the suppression rate reached 60. 8% when 2 000 UCMSCs were plated. Conclusions UCMSCs had an immunomodulatory function. UCMSCs may suppress the transformation of lymphocytes from allogeneic UCB and the suppression function umbilical cord; mesenchymalslem cell; immnnomodulatory suppression关键词
脐带/间充质干细胞/免疫抑制Key words
umbilical cord/ mesenchymalslem cell/ immnnomodulatory suppression分类
医药卫生引用本文复制引用
张玉琳..人脐带间充质干细胞抑制同种异体脐血淋巴细胞的转化[J].基础医学与临床,2012,32(8):930-934,5.基金项目
宁波天一职业技术学院2010年自然科学研究项目(201001) （201001）
