基础医学与临床2012，Vol.32Issue(8)：953-955,3.
一种改良的蛋白质电泳考马斯亮蓝G-250染色方法
An improved method for protein staining with CBBG-250 following electrophoresis
摘要
Abstract
Objective To establish a simple and less toxic method for protein staining following SDS-PAGE. Methods After electrophoresis, the gel was fixed temporarily, and then stained with the Coomassie Brilliant Blue G-250 (CBBG-250) solution containing aminosulfonic acid. De-staining was not required. Results The sensitivity of this method was higher than that of the classical CBBR-250 staining method. Moreover, toxic solvents such as metha-nol, though commonly used in the staining and de-staining solutions, were eliminated. Conclusions The improved method has a series of advantages including high sensitivity, clear background, dye-saving, direct color development without de-staining, and having a safe and less toxic recipe.关键词
电泳/聚丙烯酰胺/蛋白质染色/考马斯亮蓝Key words
electrophoresis/polyacrylamide/protein staining/Coomassie Brilliant Blue分类
生物科学引用本文复制引用
张瑶,刘芳,张页..一种改良的蛋白质电泳考马斯亮蓝G-250染色方法[J].基础医学与临床,2012,32(8):953-955,3.基金项目
国家自然科学基金(31171296) （31171296）
