摘要
Abstract
Objective To investigate the effect of sodium ferulate (SF) on apoptosis of vascular endothelial cells induced by oxidized low density lipoprotein (ox-LDL) in vitro. Methods Human umbilical vein endothelial cells (HUVEC) cultured in vitro were assigned into five groups: the control group, Ox-LDL (50mg/L) group, SF (5μmol/L) group, SF (l0μmol/L) group and SF (20(μmol/L) group. SF in the said concentration was added to the latter 3 groups, respectively, and they were then cultured for one hour, followed by the addition of ox-LDL (50mg/L). The effect of SF on HUVEC was assessed by MTT, and that on the ox-LDL induced HUVEC apoptosis was assessed with Hochest 33258 nucleus fluorescent staining and flow cytometer. Results A certain concentration of SF may increase the survival rate of HUVEC after the treatment with ox-LDL, thus it protects the HUVEC from injury. SF concentration below 40μmol/L was the optimal concentration in the protection of the cells from injury. With Hochest 33258 staining, the nucleus in ox-LDL group showed a dark blue fluorescence, with the appearance of typical morphological features of apoptosis, such as karyopyknosis and apoptotic body, etc. In SF (20μmol/L) group, the nuclei returned to its normal morphology. Flow cytometry revealed that the apoptosis ratio in low, medium and high concentration of SF groups (25.21% ± 4.07%, 12.45% ± 2.65% and 10.85% ± 1.23%, respectively) was obviously lower than that in ox-LDL group (31.14% ± 4.29%, P<0.05), and a dose-dependent relation was observed. Conclusion Sodium ferulate may protect the ox-LDL induced HUVEC from injury and decrease apoptosis of vascular endothelial cells.关键词
阿魏酸钠/细胞凋亡/内皮细胞Key words
sodium ferulate/ apoptosis/ endothelial cells分类
医药卫生
