解放军医学杂志2012，Vol.37Issue(6)：585-588,4.
药根碱对脂肪细胞糖脂代谢的影响及其机制研究
Effect of jatrorrhizine on the glucolipid metabolism in adipocyte and its mechanism
摘要
Abstract
Objective To investigate the effect of jatrorrhizine, extracted from the rhizome of Coptis chinensis, on the glucose up-take and fatty acid oxidation in the 3T3-L1 adipocyte. Methods Jatrorrhizine in different concentrations (265.65, 53.75, 10.75, 2.15 and 0.45u,mol/L) was used in culture of the 3T3-L1 adipocytes for different periods (12, 24, 48 and 72 hours), and the cytotoxicity of jatrorrhizine was then determined by MTT assay. The uptake of glucose mediated by insulin was assessed by 3H-labeled deoxyglucose, and fatty acid oxidation in co-cultured 3T3-L1 adipocyte was measured using 14 C-labeled palmitic acid. The expression of PPARs gene was detected by real-time PCR, and the expression of PPARs protein was detected by Western blotting. Jatrorrhizine was replaced with PBS solution for all the control experiments. Results The optimal active concentration of jatrorrhizine was 0.45|xmol/L, and the preferable reaction time was 48 hours. Compared to PBS solution, the in vitro fatty acid oxidation in 3T3-L1 adipocyte was promoted by jatrorrhizine, so were the genie and protein expressions of PPARα and PPARp (P<0.01 or P<0.05). The impact on the PPAR 7, however, was negative. Conclusion Jatrorrhizine can promote the fatty acid oxidation in 3T3-L1 adipocyte, which may be attributable to the up-regulation of PPAR α and PPAR |3 levels in 3T3-L1 adipocyte.关键词
药根碱/3T3-L1细胞/碳水化合物代谢/脂类代谢/过氧化物酶体增殖物激活受体Key words
jatrorrhizine/ 3T3-L1 cells/ carbohydrate metabolism/ lipid metabolism/ peroxisome proliferator-activated receptors分类
医药卫生引用本文复制引用
王慧,陈刘,姜友昭,陈兵..药根碱对脂肪细胞糖脂代谢的影响及其机制研究[J].解放军医学杂志,2012,37(6):585-588,4.基金项目
国家科技支撑计划地方专项(2007BA14B03) （2007BA14B03）
