解放军医学杂志2012，Vol.37Issue(7)：676-680,5.
F10基因过表达对A549细胞致瘤性的影响
Effects of gene F10 over-expression on the tumorigenicity of A549 cells
摘要
Abstract
Objective TTo explore the effects and mechanism of gene P10 over-expression on the tumorigenicity of AS49 cells in nude mUe. Methods Eighteen SPF nude mice (4-5weeks of age) were randomly equally divided into Che three groups: A549-WT (vaccination witK wild-type strain AS49), Mock-A549 (vaccination with conttollui cells Mock-A549 transfected fey blank vectors) and F10+A549 (vaccination with F10+A549 cell" which overexpressed F10 gene) according to their vaccination and then revaccinated into the subcutaneous tissue of the back with cell suspension (0.1ml with 5 x 10' cells in each mouse). After vaccination, the mice were observed and weighed once every 3-4 days, then the tumor formation time was recorded, the tumor growth curve drawn and tumor formation rate calculated. The mice were sacrificed ind the subcutaneous tumor tissues were paraffin embedded and sectioned, and stained with HE for histopathological examination S weeks after the vaccination. Meanwhile, tie expressions of Caspase-3, Bax and Bd-2 in the tumor tissue were detected by imronnohirtochemistry. Reoolts The tumor formation time was significantly different (p=1 3.523, P=0.000) between the groups of A549-WT (12.0 ± 14d), Mock-A549 (11.7 ± 1.0d) and F1O+A549 (8.5 ± 1.4d), and it was longer in the groups A549-WT and Mock-AS49 than that in F10+A549 group (P<0.05). while there was no significant difference between A549-WT and Mock-A549 groups (P=0.660). General observation of subcutaneous tumor tissues revealed that the tumor volume was increased more significantly in the F10+AS49 group compared with that of the groups A549-WT and Mock-A549. The growth rate of subcutaneous tumor in the F10+A549 group was increased more significantly compared with groups A549-WT and Mock-AS49 (P<0.05), while there was no significant difference between A549-WT and Mock-AS49 (P>0.05). Pathological examination revealed that the tumor tissues in the groups A549-WT and Mock-A549 harbored more necrotic cells, with structure of homogeneously red stained and amorphous material. In F10+AS49 group, the number of necrotic cell was less, and cell proliferation was obvious in the edge of tumor tissues, in which, microvessels were found. In groups A549-WT and Mock-A549, the expression of Bcl-2 was rarely observed, while it was obvious in F10+AS49 group, with positive cells diffusely distributed. In groups A549-WT and Mock-A549, there was strong the expression of both Bax and Caspase-3, especially in the AS49-WT group. But the expressions of Bax and Caspase-3 were weak in F10+A549 group. Conclusion F10 gene may down-regulate expressions of Caspase-3 and Bax, and up-regulate expression of Bd-2, which further enhance the tumorigenicity of lung cancer cell line A549 in nude mice.关键词
肺肿瘤/F10基因/A549细胞/致癌性试验Key words
lung neoplasms/ gene F10/ A549 cells/ carcinogenicity tests分类
医药卫生引用本文复制引用
宋亚丽,张弓,庞战军,朱秀兰,杨晓萍,李雅芳,全松,邢福祺..F10基因过表达对A549细胞致瘤性的影响[J].解放军医学杂志,2012,37(7):676-680,5.基金项目
国家自然科学基金(30672234) （30672234）
