解放军医学杂志2012，Vol.37Issue(8)：756-759,4.
成纤维细胞生长因子7、10及其受体2Ⅲb在小鼠肾脏发育中的表达
Expression of FGF7, FGF10 and FGFR2Ⅲb in the process of development of mouse kidney
摘要
Abstract
Objective To observe the expressions of fibroblast growth factor 7 and 10 (FGF7, FGF10) and fibroblast growth factor receptor 2lIIb (FGFR2lIIb) in kidney tissue of mice during different development stages, and investigate the relationship between FGF7, FGF10, FGFR2flIb and renal development. Methods The kidneys of mice of fetal age 12, 14, 16 and 18d and neonatal age of 1, 3, 7, 14, 21 and 42d were selected. The expressions of FGF7, FGF10 and FGFR2HIb were determined with immunohistochemistry and Western blotting, and qualitatively and quantitatively analyzed with stereological method. Results Immunohistochemical staining showed that FGF7, FGF10 and FGFR2 IHb were weakly expressed in the ureteric buds at the fetal age of 12d (E12d), and then; along with the renal development; they expressed mainly in distal tubules and collecting ducts. FGF 10 was expressed in the proximal convoluted tubule, but not in the proximal straight tubule, while no expression of FGF7 or FGFR2HIb was found in the proximal tubule* No expressions of FGF7, FGF 10 and FGFR2lHb were found in the metanephrogenic tissue and corpuscular renis in each development stage- Western blotting and stereological analysis revealed that the expressions of FGF7, FGF 10 and FGFR2 DI b increased obviously from E14 to N7, and then decreased gradually from N7 to N42. Conclusions FGF7, FGF10 and FGFR2fflb might play an important role in the renal development. FGF 10 plays its role mainly in proximal convoluted tubule, distal tubule and collecting duct, while FGF7 and FGFR2 Ⅲb play their roles dominantly in distal tubule and collecting duct关键词
成纤维细胞生长因子/受体,成纤维细胞生长因子,2型/肾Key words
fibroblast growth factors/ receptor, fibroblast growth factor, type 2/ kidney分类
医药卫生引用本文复制引用
王堃,李佳,田娟..成纤维细胞生长因子7、10及其受体2Ⅲb在小鼠肾脏发育中的表达[J].解放军医学杂志,2012,37(8):756-759,4.基金项目
辽宁省教育厅科学技术研究项目(L2010274) （L2010274）
辽宁医学院青年科技启动基金项目(Y2010Z005) （Y2010Z005）
