吉林农业大学学报2012，Vol.34Issue(4)：423-427,442,6.
苹果梨果实着色相关酶基因片段克隆及表达分析
Cloning and Expression Analysis of Gene Fragments of Related Fruit-Coloring Enzymes in Pingguoli
摘要
Abstract
In the study, fragments of genes coding related fruit-coloring enzymes(PAL, CHI and UFGT) in Pingguoli were cloned through RT - PCR, and their expressions in different developmental stages were analyzed using Semi-Quantitative RT - PCR. The results suggested that the percentages of the same amino acids (including the similar ones) that compose PAL, CHI and UFGT in Pingguoli and other plants were all about 90% , and amino acid sequence of Pingguoli showed closer clustering relationships with those of Pyrus bretschneideri Rehd, P. pyrifolia Nakai and Pyrus communis respectively. Besides, bagging inhibited the expressions of PAL, CHI and UFGT genes, while the expression levels of these genes increased after the bag was taken off.关键词
苹果梨/PAL基因/CHI基因/UFGT基因/半定量RT- PCRKey words
Pingguoli/PAL gene/CHI gene/UFGT gene/semi-quantitative RT- PCR分类
农业科技引用本文复制引用
孙百灵,曲柏宏,李伟,王旭..苹果梨果实着色相关酶基因片段克隆及表达分析[J].吉林农业大学学报,2012,34(4):423-427,442,6.基金项目
国家自然科学基金项目(30860169,31160389) （30860169,31160389）
