计量学报2012，Vol.33Issue(4)：377-380,4.DOI:10.3969/j.issn.1000-1158.2012.04.20
柱前衍生化高效液相色谱-荧光检测法测定水产品中氨苄青霉素和阿莫西林残留
Determination of Ampicillin and Amoxicillin Residues in Aquatic Product Using Reversed-phase High Performance Liquid Chromatography-fluorescence Detection with Pre-column Derivatization
摘要
Abstract
A methd is developed for determination of ampicillin and amoxicillin residues in aquatic product using reversed-phase high performance liquid chromatography-fluorescenee detection with pre-column derivatization. The sample extracts are cleaned up by solid-phase extraction cartridge and then derivatized with derivative agent. The results show that the calibration graphs is linear in the concentration rang of 0. 001 - 0. 1 mg/kg( r = 0. 999 9 ) . The detection limit and quantification limit are 0. 001 mg/kg(5/N = 3) and 0. 003 mg/kg(S/N= 10) for ampicillin, 0.005 mg/kg(S/N =3) and 0. 015 mg/kg( S/N = 10) for amoxicillin. The average recoveries are between 76. 3% - 96. 8% with relative standard deviations(RSDs) of 0. 63% -6. 71% (n =6). The method is simple,rapid,sensitive and reproducible,it can be used for the routine analysis of the ampicillin and amoxicillin residues in aquatic product.关键词
计量学/氨苄青霉素/阿莫西林/高效液相色谱法Key words
Metrology/ Ampicillin/ Amoxicillin/ High performance liquid chromatography引用本文复制引用
王萍亚,周勇,黄鹂,赵华,薛超波,许镇坚,方益,陈忠伟..柱前衍生化高效液相色谱-荧光检测法测定水产品中氨苄青霉素和阿莫西林残留[J].计量学报,2012,33(4):377-380,4.基金项目
质检公益性行业科研专项(201210060) （201210060）
国家质检总局科技计划项目(2011QK164) （2011QK164）
