解剖学杂志2012，Vol.35Issue(2)：145-148,封2,5.
SiRNA沉默HLA-A2表达的人骨髓间充质干细胞对人异体T淋巴细胞分泌功能及其诱导成骨能力的影响
Effects of RNA silenced HLA-A2 in hBMSCs on secretory function of human allogeneic T lymphocytes and osteogenic ability of hBMSCs
摘要
Abstract
Objective; To explore the secretory function of human allogeneic T lymphocytes cocultured with human bone marrow derived mesenchymal stem cells (hBMSCs), in which the HLA-A2 gene was silenced using RNA interference, and, to observe the influence of this RNA interference on osteogenic ability of hBMSCs. Methods; The hBMSCs were harvested and identified. And, human allogeneic T lymphocytes stimulated by PHA were cocultured with 1st to 3rd passaged hBMSCs before and after transferred with siRNA targeting HLA-A2. 72 h later, the levels of IFN-y and IL-2 secreted from human allogeneic T lymphocytes were detected using ELISA. Subsequently, the osteogenic induction of hBMSCs with and without RNA interference was performed in vitro. The osetogenic ability was judged by alkaline phosphatase (ALP) staining and Von-Kossa staining. Results-. The cultured hBMSCs were CDt4 and CD166 positive. The RNA interference targeting HLA-A2 induced a significant down-regulation of HLA-A2 in hBMSCs. The levels of IFN-y and IL-2 were decreased from human allogeneic T lymphocytes cocultured with transferred or non-transferred hBMSCs, especially with transferred ones. No difference was found both in ALP and Von-Kossa staining. Conclusion: The HLA-A2 silence in hBMSCs has stronger inhibition on IFN-y and IL-2 secreted from human allpgeneic T lymphocytes than non-transferred hBMSCs, but has no effects on its ostogenic ability.关键词
骨髓间充质干细胞/RNAi技术/T淋巴细胞/分泌功能/成骨能力Key words
mesenchymal stem cells/ RNAi technology/ T lymphocytes secretory function/ osteogenetic ability引用本文复制引用
单伟,包翠芬,郑仕杰,刘雷,王岩,秦书俭..SiRNA沉默HLA-A2表达的人骨髓间充质干细胞对人异体T淋巴细胞分泌功能及其诱导成骨能力的影响[J].解剖学杂志,2012,35(2):145-148,封2,5.基金项目
辽宁省教育厅基金(LT2010061) （LT2010061）
辽宁省自然科学基金(20102138) （20102138）
辽宁医学院青年科技启动基金(Y2010Z004) （Y2010Z004）
