解剖学杂志2012，Vol.35Issue(2)：171-173,185,4.
上皮钙黏蛋白在肺癌组织中的表达及其基因点突变
Expression of E-cadherin in lung cancer and its point mutation
摘要
Abstract
Objective: To investigate the expressions of E-cadherin in pulmonary adenocarcinoma and adenosquamous carcinomas and explore the relationship between its point mutation and genesis of lung cancer. Methods-. The expressions of E-cadherin in 31 cases of lung cancer tissues and 13 cases of adjacent normal tissues were examined by immunohistochemistry. Point mutations of E-cadherin on chromosome 7 and 9 were detected in 25 pairs of lung cancer tissues and adjacent normal tissues with PCR method. Results-. The positive rate of E-cadherin in lung cancer was significantly lower than that in adjacent normal tissues (26. 56%). Compared with well-differentiated and medium-differentiated lung cancer, the expression of E-cadherin in low-differentiation type was decreased by 40%. Compared with the non-metastasis group, the expression of E-cadherin in the metastasis group was decreased by 18. 5%. The point mutations on chromosome 7 of E-cadherin were both 52% in lung cancer tissues and adjacent normal tissues. The point mutations on chromosome 9 were 64% and 60% respectively. There was no difference between two groups. Conclusion-, The expression of E-cadherin plays an important role in the genesis, development and metastasis of lung cancer. The mutation of E-cadherin does occur in lung cancer, but it is not the only factor in determining its down-regulation.关键词
肺癌/上皮钙黏蛋白/突变/免疫组织化学/PCRKey words
lung cancer/ E-cadherin/ mutation/ immunohistochemistry/ PCR引用本文复制引用
郝立宏,赵瑾瑶,宫琳琳,宋阳,杨晓燕,马坚伟..上皮钙黏蛋白在肺癌组织中的表达及其基因点突变[J].解剖学杂志,2012,35(2):171-173,185,4.基金项目
辽宁省教育厅重点实验室项目(LS2010058) （LS2010058）
