扬州大学学报（农业与生命科学版）2012，Vol.33Issue(1)：10-13,4.
人CD46基因启动子的克隆和启动特性研究
Cloning of human CD46 gene promoter and its promoting characteristic
摘要
Abstract
To study the promoting characteristic of human CD46 gene promoter, its DNA fragment was amplified by PCR using the template of the genomic DNA of HeLa cells. Sequence analysis revealed that the PCR product was 99. 9% homologous to a DNA fragment in the 5' end of hCD46 gene published in 2006. This PCR cloned hCD46 gene promoter was used to construct an eukaryotic expression reporting vector by using it to replace the CMV promoter in pcDNA3 EG-FP. In order to enhance the expression level of interest genes, the 2nd intron of the gene encoding the rabbit /3-globulin (RG1) was placed between the hCD46 gene promoter and the reporter gene of EGFP. The resultant vectors were trans-fected into 2 murine cell lines, CHO and SP2/0, respectively. FACS analysis demonstrated that the self-cloned hCD46 gene promoter could direct EGFP gene to express) the relative expression levels of EGFP in the 2 cell lines were similar to that of hCD46 in human homologous tissues. These data indicate that the self-cloned hCD46 gene promoter maintained its original promoting characteristic, thus could be used to establish hCD46 transgenic animals mimicking the expression properties of hCD46.关键词
人CD46基因启动子/EGFP/启动特性/淋球菌Key words
human CD46 gene promoter/ EGFP/ promoting characteristic/ Syphilus分类
生物科学引用本文复制引用
李国才,刘双喜,于峰,杜庆辉,王劲松,潘兴元,季明春,焦红梅..人CD46基因启动子的克隆和启动特性研究[J].扬州大学学报（农业与生命科学版）,2012,33(1):10-13,4.基金项目
国家自然科学基金资助项目(31172299、31100652) （31172299、31100652）
江苏省自然科学基金资助项目(BK2010039、BY2009140) （BK2010039、BY2009140）
