扬州大学学报（农业与生命科学版）2012，Vol.33Issue(2)：1-5,10,6.
猪CD151重组抗原表达及其免疫血清的PRRSV感染抑制作用
Expression of recombinant porcine CD151 and inhibitory effect of the antiserum on PRRSV infection
摘要
Abstract
The coding sequence of the extracellular domain of pig CD151 was amplified using RT-PCR and subcloned into an expression vector containing the IgV sequence of T lymphocyte-associated antigen 4. Expression of the fusion protein in the recombinant E. coli was detected using SDS-PAGE and the soluble protein was obtained by denaturation and re-naturation of the inclusion bodies. After twice immunization of mice with the recombinanl protein, the immune serum was titrated using EI.ISA and its specificity to pig CD151 was confirmed by Western-blotting. The inhibitory effect of the immune serum on porcine reproductive and respiratory syndrome virus (PRRSV) infection of Marc-145 cells was investigated by treating the virus or cells. The results showed that the recombinant E. coli expressed an expected 32 ku fusion protein and that the soluble protein was obtained with high purity. The immune serum had an antibody titer of 1;17 000 and could detect an expected 29 ku protein in pig alveolar macrophages could be detected. A dose-dependent inhibitory effect on PRRSV infection was detected after treatment of cells or virus with the immune serum. These data indicated that the antiserum against pig CD151 can inhibit PRRSV infection of MARC-145 cells by binding to the cells or virus.关键词
猪CD151/重组抗原/免疫血清/PRRSV/感染抑制Key words
porcine CD151/recombinant antigen/antiserum/PRRSVt inhibitory effect分类
农业科技引用本文复制引用
张钰,孙怀昌,黄燕燕,张鑫宇,夏晓莉..猪CD151重组抗原表达及其免疫血清的PRRSV感染抑制作用[J].扬州大学学报（农业与生命科学版）,2012,33(2):1-5,10,6.基金项目
国家转基因生物新品种培育重大专项(2009ZX08010-019B) （2009ZX08010-019B）
江苏高校优势学科建设工程项目(PAPD) （PAPD）
禽类预防医学教育部重点实验室“教育部创新团队”项目(IRT0978) （IRT0978）
