扬州大学学报（农业与生命科学版）2012，Vol.33Issue(2)：18-22,5.
海洋细菌抗肿瘤活性菌株的筛选与鉴定
The screening and identification of marine bacteria with antitumor activity
摘要
Abstract
The antitumor activity of some marine bacterial isolates was investigated to provide potential active strains as well as novel active compounds for the antitumor drug development. In this study, by using MTT assay, we screened for the antitumor activity from some marine bacterial strains which were isolated from San Juan Island. The results showed that,among 251 tested bacterial isolates, 54 isolates (about 21.5%) showed better antitumor activity. Their inhibition rates against HeLa cell at the concentration of 100 μg · mL-1' were higher than 50%. And 7 of them showed stronger antitumor activity with the inhibitory rate higher than 85%. Among all the active strains, bacterium No. 657 showed the most remarkable antitumor activities. The antitumor activities (IC50) of its metabolies extracted from liquid fermentation and solid-state fermentation were 36. 53 and 3.19 μg · mL-1, respectively. This strains was identified as Serratia protea-maculans, according to its morphological observation and 16S rDNA sequence analysis. Our results indicated that marine microorganisms are an important source of antitumor substances. Isolate No. 657 might be a good antitumor active strain and the solid-state fermentation should be better for its active metabolites production.关键词
海洋细菌/抗肿瘤活性/MTT法/抑制率Key words
marine bacteria/antitumor activities/MTT assay/the inhibition rate分类
生物科学引用本文复制引用
王雪玲,缪莉,董昆明,周晓见,靳翠丽,钱晓晴,马君千..海洋细菌抗肿瘤活性菌株的筛选与鉴定[J].扬州大学学报（农业与生命科学版）,2012,33(2):18-22,5.基金项目
国家自然科学基金资助项目(41076097、41006097、41106113) （41076097、41006097、41106113）
新药研究国家重点实验室开放基金项目(SIMM1106KF-09) （SIMM1106KF-09）
