扬州大学学报（农业与生命科学版）2012，Vol.33Issue(2)：23-28,6.
鸡胚原始生殖细胞生物学特性鉴定及相关基因表达研究
Study on identification of chicken primordial germ cells and expression of specific genes
摘要
Abstract
Primordial germ cells CPGCs) were separated by EDTA-trypsin collection methods and cultured in vitro. The characteristics of the 5-6th passage of chicken PGCs was analyzed with alkaline phosphatase (APK) staining and immunofluorescent labeling of stage specific embryonic antigen-1(SSEA-1). was a promising the expression of germ cell differentiation associated genes on PGCs was detected by RT-PCR, The results showed that positive labeling of AKP and SSEA-1 suggested PGCs maintained undifferentiated state. The specific marker genes of PGCs Blimp-1,Oct-4, Dazl, Nanog, GDF3 and CVH were detected, but Strati was not detected in obtained PGCs. In vitro cultured PGCs have the characteristics of pluripotent stem cells, at the same time the specific marker genes of PGCs were expressed, thus we suc cessfully obtained the chicken PGCs, which was a promising a source for in vitro culture and identification of chicken PGCs.关键词
鸡/胚胎原始生殖细胞/体外培养/生物学特性/鉴定Key words
chicken/primordial germ cells/in vitro/biological characteristics/identification分类
农业科技引用本文复制引用
施青青,孙敏,郑蒙蒙,王丹,黄晓梅,张亚妮,李碧春..鸡胚原始生殖细胞生物学特性鉴定及相关基因表达研究[J].扬州大学学报（农业与生命科学版）,2012,33(2):23-28,6.基金项目
国家自然科学基金资助项目(30871791) （30871791）
江苏省研究生培养科研创新计划项目(2010-05) （2010-05）
江苏省“六大人才高峰”项目(NY201001),江苏省高校自然科学重大基础研究项目(08KJA230001) （NY201001）
高等学校博士学科点专项科研基金资助项目(20103250110006) （20103250110006）
