扬州大学学报（农业与生命科学版）2012，Vol.33Issue(2)：58-61,4.
提高甘蓝型油菜下胚轴愈伤组织诱导率的研究
Studies on the enhancing callus induction ratio from hypocotyl explants of Brassica napus L
摘要
Abstract
In the past decades, the plant genetic engineering technology in improving the yield and quality of Brassica na pus L.had been greatly developed. Establishing an efficient and stable regeneration system of explant is one of the important segments of genetic engineering breeding. In this study, hypoeolyis of Brassica napus L. were used as the ex-plant, and the callus induction ratio was analyzed under different gcnotypes, seedling ages and combinations of various hormones. The results showed that Yangyou 6 hypocotyl explant had the largest contribution on the callus formation under the same tissue culture conditions, and the best conditions of callus formation was the 5 d age seedling, pre-culturing for 3 d, culturing in the medium containing 0.1 mg· L-1 2,4-D, 1.0mg· L-1 6-BA and 5.0mg· L-1 AgNO3.关键词
甘蓝型油菜/下胚轴/组织培养/愈伤组织Key words
Brassica napus/hypocotyl/tissue culture/callus分类
农业科技引用本文复制引用
杜坤,范芸,吴冕,周晓燕,高亚楠,王幼平..提高甘蓝型油菜下胚轴愈伤组织诱导率的研究[J].扬州大学学报（农业与生命科学版）,2012,33(2):58-61,4.基金项目
国家自然科学基金资助项目(31171581) （31171581）
江苏省研究生创新项目(CXZZ12-0897) （CXZZ12-0897）
