江西农业大学学报2012，Vol.34Issue(2)：339-344,6.
GM-CSF与生长抑素融合表达质粒对小鼠肌肉组织GHR和IGF-ⅠmRNA表达的影响
GHR and IGF-Ⅰ mRNA Expression in Muscle Tissue of Mice after Immunization with Fusion-Expression Plasmid Contained GM-CSF and SS
摘要
Abstract
A total of 70 mice at 22 days age were randomized to 7 groups to detect GHR and IGF -1 mRNA expression in muscle tissues by semi - quantitative RT - PCR, and β - actin was served as the control. All mice of 7 groups were given twice immunizations at intervals of 2 weeks. Group 1 as the control was immunized with 0.85 % salt solution. Group 2-4 were given muscular injections (i m. ) with pcS/2SS ( Group 2) , pGM - CSF/SS ( Group 3) and pGM - CSF + peS/2SS ( Group 4) , respectively, while Group 5 -7 by oral administration with pcS/2SS (Group 5) , pGM - CSF/SS ( Group 6) and pGM - CSF + pcS/2SS ( Group 7) delivered by attenuated Salmonella. The results were as follows; GHR mRNA expression levels of Group 3,5 and 6 were higher than that of Group 1 and 7 (P < 0.05). IGF - I mRNA expression levels of Group 5 and 6 were higher than that of Group 1 ,2 ,4 and 7 ( P < 0. 05 ) . GHR and IGF - I mRNA expression levels of pGM - CSF/ SS were higher than that of co - immunization of pGM - CSF + pcS/2SS. The results also showed that GHR and IGF -1 nmRNA expression levels by the oral administration were largely higher than that of the muscular injection with the same DNA vaccine. These results suggest that GM - CSF can enhance immune effect of SS DNA vaccine and increase GHR and IGF -I mRNA expression in muscle tissue of mice. The immune effect of pGM - CSF/SS is superior to that of pGM - CSF + pcS/2SS. The immune response of oral administration is higher than that of the muscular injection.关键词
粒细胞-巨噬细胞集落刺激因子/生长抑素(SS)/DNA疫苗/生长激素受体/胰岛素样生长因子-1/mRNA表达Key words
granulocyte - macrophage colony -stimulating factor (GM-CSF) / somatostatin (SS) / DNA Vaccine/ growth hormone receptor (GHR) / insulin-like growth faclor-I (IGF- I) / mRNA Expression分类
生物科学引用本文复制引用
舒邓群,朱晓华,吴红翔,王开云,曾检华,魏金刚,胡豆..GM-CSF与生长抑素融合表达质粒对小鼠肌肉组织GHR和IGF-ⅠmRNA表达的影响[J].江西农业大学学报,2012,34(2):339-344,6.基金项目
江西省教育厅科技项目(GJJ08187)和江西农业大学博士启动基金 （GJJ08187）
