江西农业大学学报2012，Vol.34Issue(3)：522-527,6.
五角枫组织脱分化与芽增殖的初步研究
Dedifferentiation and Shoot Proliferation of Acer mono Maxim
摘要
Abstract
Aiming at the fact that the vegetative propagation of the Acer mono Maxim, is difficult, this study was conducted to deal with the dedifferentiation and shoot proliferation of Acer Mono Maxim, and the organogenesis of the plantlet . Seeds of Acer mono Maxim, were sterilized and cultivated in MS medium. Leaves, cotyledons and roots from seedlings were used for the dedifferentiation. The result showed that leaves had the highest induction rate of callus and the best medium for callus inducing was MS+6-BA 0.5 mg/L+2,4-D 1.0 mg/L+ NAA0.5 mg/L; the optimal proliferation medium for callus was MS+6-BA 1.0 mg/L+ 2,4-D 0.5 mg/L+ NAA 0.5 mg/L+ TDZ 1.5 mg/L. Appropriate medium for shoot proliferation was MS+ TDZ 0.04 mg/L, and that for the tissue culture rooting was 1/2MS+IBA1.5 mg/L.The regenerated plants of Acer mono Maxim were obtained for the first time through the dedifferentiation and redifferentiation process and a foundation for the techniques of propagation of Acer mono Maxim was established.关键词
五角枫/组织脱分化/器官再生/芽增殖Key words
Acer mono Maxim./ dedifferentiation/ organogenesis/ shoot proliferation分类
农业科技引用本文复制引用
聂鹤云,石琨,郑彩霞..五角枫组织脱分化与芽增殖的初步研究[J].江西农业大学学报,2012,34(3):522-527,6.基金项目
国家自然科学基金项目(30671655)和山西省杨树局合作项目 （30671655）
