江西农业学报2012，Vol.24Issue(4)：153-156,4.
猪hepcidin基因质粒载体的构建及其在毕赤酵母中的表达
Plasmid Vector Construction of hepcidin Gene in Pig and Its Expression in Pichia pastoris
摘要
Abstract
In order to construct Pichia pastoris secretive expression vector of hepcidin and realize its eukaryotic expression, hepcidin, an iron - metabolism regulating gene, was amplified from pig' s liver by RT - PCR, and the recombinant expression plasmid pGAPZaA - hepcidin was constructed by connecting the obtained hepcidin with eukaryotic expression vector pGAPZaA through ligase. This plasmid vector was transformed into Pichia pastoris by high - voltage electroporation, and the target colonies were selected by Zeo-cin and PCR detection. The results showed that hepcidin gene was correctly inserted into expression vector pGAPZaA and integrated into the genome of this yeast, and the P. pastoris recombinant expression plasmid of hepcidin was successfully constructed.关键词
猪/hepcidin/毕赤酵母/基因表达Key words
Pig/ hepcidin / Pichia pastoris/ Gene expression分类
生物科学引用本文复制引用
狄元冉,卢敏,袁林,常娟,尹清强,左瑞雨,郑秋红,王潇,刘俊熙..猪hepcidin基因质粒载体的构建及其在毕赤酵母中的表达[J].江西农业学报,2012,24(4):153-156,4.基金项目
郑州市科技创新团队(112PCXTD339). （112PCXTD339）
