昆虫学报2012，Vol.55Issue(4)：395-402,8.
优雅蝈螽精子形成过程中F-肌动蛋白的动态变化
Dynamics of F-actin during spermiogenesis in Gampsocleis gratiosa ( Orthoptera: Tettigoniidae)
摘要
Abstract
In order to understand the dynamics of F-actin during spermiogenesis in Gampsocleis gratiosa Brunner von Wattenwyl, we demonstrated the cellular localization of F-actin during spermiogenesis using differential interference contrast microscopy and immunofluorescence microscopy, and observed the ultrastructure of the acrosomal complex using transmission electron microscopy. The results show that F-actin is detected in the subacrosomal space at the early spermatid stage, with shape from globular to rod-cone-like. At the middle spermatid stage, F-actin is detected in the subacrosomal space and two sides of the nuclear forepart, and looks like an inverted Y. At the late spermatid stage, F-actin looks like an arrow only in the extension wings of the acrosomal complex. F-actin was depolymerized and disappeared, only being located at subacrosomal region at the middle spermatid stage. The results suggest that the dynamics of F-actin is associated with the shape of spermatid nucleus and sperm head, and the acrosomal architecture and nuclear morphogenesis are supported by a dynamic F-actin skeleton during spermiogenesis. In addition, we discovered some cytoplasm droplets in the tail of immature sperm. Immunocytochemieal and ultrastruetural analysis indicated that these cytoplasm droplets contain abundant F-actin. The F-actin of cytoplasm droplets can be involved in expelling redundant cytoplasm and organelles during spermiogenesis. This study on the dynamics of F-actin provides the foundation to clarify the function and mechanism of cytoskeletal proteins during insect spermiogenesis.关键词
优雅蝈螽/F-肌动蛋白/精子形成/顶体复合体/细胞质微滴/细胞器Key words
Gampsocleis gratiosa/ F-actin/ spermiogenesis/ acrosomal complex/ cytoplasm droplet/ organelle分类
生物科学引用本文复制引用
周娜,常岩林,王莉..优雅蝈螽精子形成过程中F-肌动蛋白的动态变化[J].昆虫学报,2012,55(4):395-402,8.基金项目
国家自然科学基金项目(31093430,31101632) （31093430,31101632）
