昆虫学报2012，Vol.55Issue(6)：703-709,7.
橘全爪螨对双甲脒的抗性选育及其P450基因的表达差异分析
Selection of resistance to amitraz and analysis of expression difference of cytochrome P450 genes in Panonychus citri (Acari: Tetranychidae)
摘要
Abstract
To evaluate the resistance risk of Panonychus citri to amitraz and clarify the relationship between expression level of cytochrome P450 gene and resistance of P. citri, resistance selection was conducted in the laboratory, and cross-resistance of the amitraz-resistant strain was studied with slide-dip method and cytochrome P450 gene expression profiles between the resistant strain and the susceptible strain were compared using RPKM (reads per kb per million reads) method. After selection with amitraz continuously for 12 generations, a resistant strain was obtained with the resistance ratio (R/S) of 26. 32 compared with the susceptible strain. The realized heritability of resistance to amitraz was 0. 148. Bioassay data showed that the amitraz-resistant strain had positive cross-resistance to spirodiclofen, diafenthiuron, propargite and azocyclotin (the R/S ratio was 16. 85, 4. 98, 2. 13, and 2. 05, respectively), but had no cross-resistance to abamectin, fenbutatin oxide, pyridaben and petroleum oil (the R/S ratio was 1. 10, 1. 21, 0. 67 and 0. 99, respectively). Gene expression difference analysis results indicated that 16 cytochrome P450 genes were up-regulated and 27 cytochrome P450 genes were down-regulated in the resistant strain. CYP389A6 [ log2ratio ( RS/SS) =11.526] and CYP389A2 [ log2 ratio(RS/SS) = -12.683] were the top up-regulated and down-regulated genes, respectively, which are very likely to be associated with the resistance of P. citri to amitraz.关键词
橘全爪螨/双甲脒/交互抗性/抗性品系/敏感品系/P450基因/基因表达差异Key words
Panonychus citri/ amitraz/ cross-resistance/ resistant strain/ susceptible strain/ P450 gene/ expression difference分类
生物科学引用本文复制引用
冉春,江高飞,刘斌,刘浩强,李鸿筠,王进军..橘全爪螨对双甲脒的抗性选育及其P450基因的表达差异分析[J].昆虫学报,2012,55(6):703-709,7.基金项目
长江学者和创新团队发展计划项目(IRT0976) （IRT0976）
国家科技支撑计划课题(2012BAD19B06) （2012BAD19B06）
柑桔学重庆市市级重点实验室开放基金资助项目 （）
