昆明医学院学报2012，Vol.33Issue(4)：16-21,6.
蒿甲醚对大鼠原位脑胶质瘤抑瘤及抗血管生成的实验研究
Study on Inhibitory Effects of Artemether on Brain Glioma Growth and Angiogenesis in SD Rats
摘要
Abstract
Objective To explore the inhibitory effect of artemether (Artrn) on glioma growth and angiogenesis in brain tumor bearing SD rats. Methods MTT (Methy thiazolyl tetrazolium) assay was used to evaluate the inhibitory effect of Artemether treatment on C6 glioma cells. 48 (24 male and 24 female) SD rats which were subcutaneous planted with SD rat C6 glioma cell (1× 1 06/ΜL) in cerebral cortex were divided randomly into 3 groups, each group had 8 rats. Blank control group:rats were orally given normal saline. Positive control group: rats were orally given Lomustine. Different artemether therapy groups: rats were given different doses of artemether. Treatment started on day 3 and continued until day 10 and SD rats were killed on day 20. Whole brain was fixed with 4% paraformaldehyde through alive left ventricle perfusion. The length- path and short- path of tumor each rat was measured by staff gauge in vertical and horizontal. Volume of tumor was calculated following formula: V (mm3) = a2bTT 6 (a:short- path, b:length- path) . Micro- vascular dense (MVD) was observed and countered under the microscopy by immunohistory chemistry. Results Inhibitory rates of Artemether at different dosages were 54.5%, 61.0%, 64.5% and 69.8%, respectively, and compared with blank control group, different experiment groups had significant difference (P = 0.000 respectively) . Micro-vascular dense in different therapy groups was significant lower than that in blank control group (P<0.05, P<0.01 respectively) and volume of brain glioma in different therapy groups was significant smaller than that in blank control group. Conclusions Artemether has inhibitory effect on brain glioma growth in SD rats. The mechanism of Artemether inhibiting brain glioma growth may be penetrating the blood- brain barrier by inhibiting angiogenesis.关键词
蒿甲醚/SD大鼠C6脑胶质瘤细胞/原位脑胶质瘤/动物模型/抑瘤及抗血管生成Key words
Artemether/ SD rat C6 glioma cell line/ Brain glioma/ Animal model/ Inhibition of glioma growth and angiogenesis分类
临床医学引用本文复制引用
伍治平,朱启顺,魏万里,黄洁,沈红梅,童书云..蒿甲醚对大鼠原位脑胶质瘤抑瘤及抗血管生成的实验研究[J].昆明医学院学报,2012,33(4):16-21,6.基金项目
云南省科技厅-昆明医学院联合基金资助项目(2009CD185) （2009CD185）
