口腔颌面外科杂志2012，Vol.22Issue(3)：174-178,5.DOI:10.3969/j.issn.1005-4979.2012.03.005
纤维蛋白胶联合几丁聚糖-胶原导管修复兔面神经损伤的效果
Assessment of Regeneration in Peripheral Nerve Repair using Fibrin Glue with Chitosan-collagen Conduit in a Rabbit Model
摘要
Abstract
Objective: This study was designed to assess the regeneration outcome of fibrin glue with chitosan-collagen conduit in peripheral nerve repair. Methods: Thirty Chinese rabbits were used in this study and their right facial nerves were traumatized. Rabbits were randomly divided into 3 groups: group I , nerve repaired by autografts, group Ⅱ, nerve repaired by fibrin glue , and group Ⅲ, fibrin glue with chitosan-collagen conduit were applied. At the end of 16 weeks after operation, gross examination of the animals, electrophysiological studies, histological studies , and image analysis were performed. Database and calculations were analyzed using analysis of variance (one-way ANOVA) with the SPSS 17.0. Results: The nerve regenerated uneventful at 16 weeks after postoperation. Chitosan-collagen conduit was seemingly degraded and restrained the formation of fibrosis. Adherence of repaired nerve with surrounding soft tissue were found more severe in group 1 than other two groups. The potential motor nerve action and the compound muscle action potential (CMAP) of regenerated nerve showed no significant difference between three groups (P>0.05). Histological study revealed various degree of fibrous hyperplasia and inflammatory reaction in the regenerative site. Fibrin glue and chitosan - collagen conduit induced effective regeneration manifested not only in low magnification of HE sections but also in high magnification of Gomori silver stain. The cross rate of myelinated fiber cross section diameter of the three groups showed differences (P<0.05), which was higher in group Ⅱ and group Ⅲ than in group I (P<0.05). There was also significant difference between group II and group I (P<0.01). The recovery rate of myelinated fiber cross section diameter of the three groups showed no significant difference (P>0.05). Conclusion: Chitosan-collagen conduit possesses excellent biocompatibility, fibrin glue may effectively regenerate peripheral nerve.关键词
纤维蛋白胶/几丁聚糖-胶原导管/周围神经/神经再生/兔Key words
fibrin glue/ehitosan-collagen conduit/ peripheral nerves/nerve regeneration/ rabbit分类
医药卫生引用本文复制引用
屈振宇,曲卫国,马卫东,陈洪涛,曲晓娟,张璐..纤维蛋白胶联合几丁聚糖-胶原导管修复兔面神经损伤的效果[J].口腔颌面外科杂志,2012,22(3):174-178,5.基金项目
大连市优秀青年科技人才基金(2006J23JH016) （2006J23JH016）
