临床输血与检验2012，Vol.14Issue(3)：193-200,8.DOI:10.3969/j.issn.1671-2587.2012.03.001
云南汉族血小板献血者HLA-A、B和HPA基因多态性的分析
Gene Frequencies of the HLA-A,B and HPA in Typed Platelet Donors of Yunnan Han Nationality
摘要
Abstract
Objective To investigate the genetic polymorphisms of HLA-A, B alleles and HPA1-17 m Yunnan Han population platelet donors and to establish typed platelet donor data bank. Methods The technique of flow polymerase chain reaction sequence specific oligonucleotide probes (PCR-SSOP) and PCR-sequence specific primers(PCR-SSP) methods were respectively adopted in genotyping HLA-A, B and HPA1-17. Samples of 1 000 unrelated platelet dornors were collected from Kunming,Yunnan province, China. The allele frequencies of HLA-Ai B and HPA1-17 were calculated, and the allele frequency distribution in this population was compared with the results from various regions. Results The number of alleles identified was 14 for HLA-A, 35 for HLA-B, respectively. The most frequent HLA-A alleles were A·02 (35.81%),A·ll<35. 42%)and A·24(17. 72%). For the HLA-B locus, the most common in descending order were B·46 (12. 87%),B· 40(60)(10. 83%) and B·15(62)(9.78%). The HPA results indicated that HPA -la, 2a, 4a-14a, 16a and 17a genes were found among the 17 HP As in every sample tested. Monomorphic HPA-4a, 7a-14a,16a and 17a were found in the samples. For HPA-1, 2, 5 and 6. a/a homozygosity was predominant with fequencies of 0. 989,0. 960,0. 990,0. 980 respectively, and none of HPA b/b was found in the samples. HPA-lb. 2b, 5b. 6b were rarely found among subjects. HPA-15 had the greatest heterozygosity with a gene frequency of 0. 392,0. 360,0. 248 for HPA15a/15a, HPA15a/15b, HPA15b/15b, respectively. HPA-3 showed the second greatest heterozygosity with a gene frequency of 0. 339,0. 467, 0.194 for HPA3a/3a, HPA3a/3b, HPA3b/3b, respectively. HPA genotype frequencies showed a good fit to Hardy-Wein-berg equilibrium. Conclusion Distribution of the allele polymorphism of HLA-A.B and HPA1-17 in Yunnan Han population is similar to that in southern Han population, and it exhibits its own characteristic. We established the local HLA- I and HPA genotyped database of platelet donors in Yunnan.关键词
HLA/HPA/基因多态性/血小板输注无效Key words
HLA HPA Genetic polymorphism Platelet transfusion refractoriness分类
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朱祥明,杨通汉,姚富柱,汤戎,赵杨,苏品璨,罗臻,涂源泉,郭萍..云南汉族血小板献血者HLA-A、B和HPA基因多态性的分析[J].临床输血与检验,2012,14(3):193-200,8.基金项目
本课题受昆明市卫生局2010立项备选项目(No.2010-17)资助 （No.2010-17）
