临床输血与检验2012，Vol.14Issue(3)：225-228,4.DOI:10.3969/j.issn.1671-2587.2012.03.010
金标法和ELISA法检测乙型肝炎病毒血清标志物的比较
Comparative Study on Colloidal Gold-labeled Method and ELISA of Testing Five Serological Markers of HBV
摘要
Abstract
Objective To investigate the sensitivity and specificity of testing HBsAg. HBsAb, HBeAg, HBeAb and HBcAb with colloidal gold-labeled method. Methods 256 serological cases were tested with both colloidal gold-labeled method and ELISA. The results of two methods were analyzed. Results All five negative results samples by ELISA were the same with colloidal gold-labeled method.but for the results HBsAg. HBeAb, HBcAb are positive)HBsAb,HBeAg are negative and HBsAg.HBeAg. HBcAb are positive; HBeAb.HBsAb are negative by ELISA. some were changed by colloidal gold-labeled method. The chi-aquare test(x2) showed that the result differences of HBsAb and HBeAg were not statistically significant with two methods (P>0.05 ). But X2 test showed the differences were significant on HBsAg,HBeAb and HBcAb with two methods. The weak positive results by ELISA always were negative by colloidal gold-labeled method. but no negative results by ELISA were found positive tested by colloidal gold-labeled method. Conclusions The specificity of testing five serological markers of HBV has no difference between two methods. but ELISA is more sensitive than colloidal gold-labeled method.关键词
金标法/ELISA法/乙型肝炎病毒/血清标志物/敏感性/特异性Key words
Colloidal gold-labeled method ELISA HBV Serological marker Sensitivity Speci-ficity分类
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丁邦胜,潘健..金标法和ELISA法检测乙型肝炎病毒血清标志物的比较[J].临床输血与检验,2012,14(3):225-228,4.
